- R73 (See other available formats)
- Regulatory Status
- Other Names
- TCR α/β, α/β TCR
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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TCR α and β are members of the immunoglobulin superfamily and components of the CD3/TCR complex expressed on most peripheral T cells, intestinal epithelial lymphocytes, and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation. The TCR α/β recognizes peptide bound to MHC class I and II.Product Details
- Rat, Cross-Reactivity: Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Rat T cell blasts & erythrocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, immunohistochemical staining of acetone-fixed frozen sections1, in vitro activation of α/β TCR+ T cells 1,2 and in vivo depletion2 of α/β TCR+ T cells . The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 201112).
(PubMed link indicates BioLegend citation)
- Hunig T, et al. 1989. J. Exp. Med. 169:73.
- Mitnacht R, et al. 1995. Eur. J. Immunol. 25:328.
- Brenan M, et al. 1997. Eur. J. Immunol. 27:3070.
- Yoshino N, et al. 2000. Exp. Anim. 49:97.
AB_313914 (BioLegend Cat. No. 201105)
- Ig superfamily, CD3/TCR complex with CD3 and TCR-α subunit, 40 and 46 kD
- Most peripheral T cells, intestinal epithelial lymphocytes, thymocytes
- Antigen recognition, T cell activation/responses
- Peptide bound-MHC class I and II
- Cell Type
- T cells, Lymphocytes, Thymocytes
- Biology Area
- Immunology, Adaptive Immunity
- Molecular Family
- Antigen References
1. Hunig T, et al. 1989. J. Exp. Med. 169:73.
2. Fangmann J, et al. 1991. Eur. J. Immunol. 21:753.
3. Mitnacht R, et al. 1995. Eur. J. Immunol. 25:328.
4. Peiters RH, et al. 1995. Biochem. Biophys. Res. Commun. 214:552.
5. Brenan M, et al. 1997. Eur. J. Immunol. 27:3070.
- Gene ID
- 24820 View all products for this Gene ID 290071 View all products for this Gene ID
- View information about TCR alpha/beta on UniProt.org
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