- OX-34 (See other available formats)
- Regulatory Status
- Other Names
- LFA-2, T11
- Mouse IgG2a, κ
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- Product Citations
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CD2, also known as LFA-2, is a member of the immunoglobulin gene family with an approximate molecular weight of 45 kD. It is expressed on thymocytes, peripheral T cells, dendritic epidermal T cells, macrophages in the spleen, and NK cells. CD2 can associate with the T cell receptor and participate in T cell activation through adhesion and signal transduction. CD2 binds to CD48, CD58, and CD59. The OX-34 antibody blocks CD48 binding to CD2. It has been reported to partially block T cell activation and inhibit the mixed lymphocyte reaction in vitro. The OX-34 antibody has also been used to deplete peripheral T cells and prevent allograft rejection in vivo.Product Details
- Antibody Type
- Host Species
- Rat T cell blasts from mixed lymphocyte reactions
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry1 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections, immunoprecipitation2, partial in vitro blocking of T cell responses3, and in vivo depletion of T cells3.
(PubMed link indicates BioLegend citation)
- Williams AF, et al.. 1987. J. Exp. Med. 165:368.
- Jefferies WA, et al.. 1985. J. Exp. Med. 162:117.
- Hirahara H, et al.. 1995. Transplantation 59:85.
AB_2228899 (BioLegend Cat. No. 201303)
- Ig superfamily, approximately 45 kD
Thymocytes, T cells, dendritic epidermal T cells, splenic macrophages, NK cells
- Adhesion, T cell activation
- CD48, CD59
- Cell Type
- Macrophages, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Jefferies WA, et al.. 1985. J. Exp. Med. 162:117.
2. Williams AF, et al.. 1987. J. Exp. Med. 165:368.
- Gene ID
- 497761 View all products for this Gene ID
- View information about CD2 on UniProt.org
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