FITC anti-rat CD11b/c Antibody

Pricing & Availability
Clone
OX-42 (See other available formats)
Regulatory Status
RUO
Other Names
Rat C3bi receptor, Rat CR3 complement receptor, ITGAM, ITGAX
Isotype
Mouse IgG2a, κ
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Product Citations
publications
OX-42_FITC_032808
LOU rat splenocytes stained with OX-42 FITC
  • OX-42_FITC_032808
    LOU rat splenocytes stained with OX-42 FITC
See FITC spectral data
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201805 100 µg $130.00
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Description

The OX-42 antibody reacts with the CR3 complement (C3bi) receptor expressed on monocytes, granulocytes, macrophages, dendritic cells, and NK cells. This antibody appears to recognize a common epitope shared between CD11b and CD11c (integrin αM and αX chains). The OX-42 antibody precipitates three polypeptides with apparent molecular weights of 160, 103, and 95 kD, respectively. This antibody has been shown to block the formation of complement-mediated rosettes and leukocyte migration.

Product Details
Technical data sheet

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat peritoneal macrophages.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of C3bi binding3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  2. Milligan CE, et al. 1991. J. Comp. Neurol. 314:125. (IHC)
  3. Robinson AP, et al. 1986. Immunology 57:239. (Block)
  4. Issekutz SE, et al. 1992. Immunology 76:655. (Block)
  5. Muehlbauer SM, et al. Am. J Pathol. 177:735. (FC) PubMed
  6. Thomas RA. et al. 2009. J Pharmacol Toxicol Methods. 60:263. PubMed
  7. Takeda Y, et al. 2011. Immunobiology. 216:1094. PubMed
  8. Furuhashi K, et al. 2013. J Am Soc Nephrol. PubMed
  9. Cheng HY, et al. 2013. Cytotherapy. 1465:608. PubMed
Product Citations
  1. Wang Y, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.006. PubMed
  2. Ben-Shoshan J, et al. 2008. J Virol. 26:2634. PubMed
  3. Lorenzo A, et al. 2009. Proc Natl Acad Sci U S A. 106:14552. PubMed
  4. Lorenzo A, et al. 2011. Blood. 117:2284. PubMed
  5. Liang H, et al. 2021. J Neuroinflammation. 18:40. PubMed
RRID
AB_313993 (BioLegend Cat. No. 201805)

Antigen Details

Structure
Integrin family member
Distribution

Monocytes, granulocytes, macrophages, dendritic cells

Function
Receptor for C3bi
Ligand/Receptor
Complement C3bi
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Cell Biology, Complement, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Robinson AP, et al. 1986. Immunology 57:239.
2. Barcaly AN. 1981 Immunology 42:593.

Gene ID
25021 View all products for this Gene ID 499271 View all products for this Gene ID
UniProt
View information about CD11b CD11c on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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