FITC anti-mouse CD62L Antibody

Pricing & Availability
Clone
MEL-14 (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat IgG2a, κ
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Product Citations
publications
MEL-14
C57BL/6 bone marrow cells stained with MEL-14 FITC
  • MEL-14
    C57BL/6 bone marrow cells stained with MEL-14 FITC
See FITC spectral data
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104405 50 µg $55.00
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104406 500 µg $185.00
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Espinosa JR, et al. 2018. Front Immunol. 9:1371. PubMed
  2. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  3. McCreedy DA et al. 2018. eNeuro. 5(4) pii: ENEURO. PubMed
  4. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  5. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  6. Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
  7. Cuburu N, et al. 2019. J Immunol. 202:1250. PubMed
  8. Nowill AE, et al. 2019. J Immunol. 203:1298. PubMed
  9. Sema Kurtulus et al. 2018. Immunity. 50(1):181-194 . PubMed
  10. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  11. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  12. Chen Z, et al. 2017. Mucosal Immunol. 10:58. PubMed
  13. Wan X, et al. 2018. Nature. 560:107. PubMed
  14. Mohammed RN, et al. 2019. Sci Rep. 4.185416667. PubMed
  15. Cutler CE, et al. 2019. Glycobiology. 29:776. PubMed
  16. Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed
  17. Kashiwakura Y, et al. 2020. Clinical & Experimental Immunology. 202(1):119-135. PubMed
  18. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  19. Prüfer S, et al. 2014. Immunobiology. 219:87. PubMed
  20. Furuya Y, et al. 2014. J Virol. 88:9166. PubMed
  21. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  22. Bonaccorsi-Riani E, et al. 2015. PLoS One. 10: 0136106. PubMed
  23. Flaherty S, Reynolds J 2015. J Vis Exp. 98: 52739. PubMed
  24. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  25. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  26. Wang Y, et al. 2020. Cell Death Dis. 1.169444444. PubMed
  27. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  28. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  29. Brigas HC, et al. 2021. Cell Reports. 36(9):109574. PubMed
RRID
AB_313092 (BioLegend Cat. No. 104405)
AB_313093 (BioLegend Cat. No. 104406)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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