FITC anti-mouse CD314 (NKG2D) Antibody

Pricing & Availability
Clone
C7 (See other available formats)
Regulatory Status
RUO
Other Names
CD314
Isotype
Armenian Hamster IgG
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Product Citations
publications
C7
C57BL/6 mouse splenocytes were stained with NK1.1 APC (clone PK136) and FITC NKG2D (clone C7, left) or Armenian Hamster IgG isotype control (clone HTK888, right).
  • C7
    C57BL/6 mouse splenocytes were stained with NK1.1 APC (clone PK136) and FITC NKG2D (clone C7, left) or Armenian Hamster IgG isotype control (clone HTK888, right).
See FITC spectral data
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115711 50 µg $90.00
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Description

NKG2D is a lectin-like type II transmembrane protein also known as CD314. It is expressed on NK cells, a subset of CD8+ T cells, γ/δ T cells and NK1.1+ T cells, as well as in vitro induced LAK cells. NKG2D serves as a stimulatory immunoreceptor to activate NK cells via the non-covalently associated DAP10 or DAP12 adaptor. Several molecules have been identified as the ligands for NKG2D, including minor histocompatibility molecule, H60, UL16-binding protein-like transcript 1 (Mult1, and a family of retinoic acid early transcript 1 (Rae1) in mice, MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs) in humans. present in both mice and humans. NKG2D ligands trigger cytokine (IFN-γ, GM-CSF, TNF-α, MIP1β and others) and granzyme release from NK cells. The C7 antibody has been reported to block NK cell killing of targets expressing NKG2D ligands and induce redirected lysis.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Soluble mouse NKG2D
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for the relevant formats) applications include: blocking1,2,4,5 and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115713 & 115714).

Application References

(PubMed link indicates BioLegend citation)
  1. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. (Block)
  2. Ho EL, et al. 2002. J. Immunol. 169:3667. (Block)
  3. Maeda M, et al. 2004. J. Immunol. 172:6115. (FC)
  4. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (Block)
  5. O'Leary JG, et al. 2006. Nature Immunology 7:507. (Block)
  6. Tang X, et al. 2006. J. Immunol. 177:7645. (FC)
Product Citations
  1. Kamizono S, et al. 2009. J Exp Med. 206:2977. PubMed
  2. Bolinger B, et al. 2013. J Immunol. 190:4162. PubMed
  3. Lin D, et al. 2016. Cancer Res . 76: 3179 - 3188. PubMed
  4. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
RRID
AB_2133291 (BioLegend Cat. No. 115711)

Antigen Details

Structure
C-type lectin
Distribution

NK and NK-T cells

Function
NK cytolytic killing of target cells expressing NKG2D ligands, costimulation NK cells
Ligand/Receptor
Minor histocompatibility molecule H60, retinoic acid early inducible 1 (RAE-1) family
Cell Type
NK cells, NKT cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Vance RE, et al. 1998. J. Exp. Med. 188:1841.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Yokoyama WM. 1998. Curr. Opin. Immunol. 10:298.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667.

Gene ID
27007 View all products for this Gene ID
UniProt
View information about CD314 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 04/18/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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