FITC anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
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Product Citations
publications
C068C2_FITC_1_072111
Thioglycollate-elicited Balb/c macrophages were fixed/pemeabilized, and then stained with CD107b (Mac-3) APC and CD206 (clone C068C2) FITC (top) or rat IgG2a, κ FITC isotype control (bottom).
  • C068C2_FITC_1_072111
    Thioglycollate-elicited Balb/c macrophages were fixed/pemeabilized, and then stained with CD107b (Mac-3) APC and CD206 (clone C068C2) FITC (top) or rat IgG2a, κ FITC isotype control (bottom).
  • C068C2_FITC_2_072111
See FITC spectral data
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141703 50 µg $115.00
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141704 200 µg $275.00
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other.

Application References

(PubMed link indicates BioLegend citation)
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  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
Product Citations
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  6. Zhang J, et al. 2014. J Immunol. 193:5149. PubMed
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  16. Chen Z, et al. 2016. Innate Immunity. 22: 419 - 432. PubMed
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  18. Cuccarese M, et al. 2017. Nat Commun. 8:14293. PubMed
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  20. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
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  27. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
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  29. Zhang F, et al. 2017. Mol Immunol. 10.1016/j.molimm.2017.06.019. PubMed
  30. Choi B, et al. 2017. Eur J Pharmacol. 10.1016/j.ejphar.2017.07.022. PubMed
  31. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  32. Xie Z, et al. 2018. J Am Heart Assoc. 7:e007442. PubMed
  33. Wang T, et al. 2018. Front Physiol. 9:209. PubMed
  34. Perrotta C, et al. 2018. Front Immunol. 9:1186. PubMed
  35. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
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  38. Li H, et al. 2019. J Immunol Res. 2018:4807145. PubMed
  39. Liang S, et al. 2019. Mol Med Rep. 19:3707. PubMed
  40. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
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  46. Ano Y, et al. 2019. Nutrients. 1.959027778. PubMed
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  48. Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed
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  56. Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
RRID
AB_10900988 (BioLegend Cat. No. 141703)
AB_10901166 (BioLegend Cat. No. 141704)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD206
Specificity Alt (DOES NOT SHOW ON TDS):
CD206
UniProt
View information about CD206 on UniProt.org

Related FAQs

Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 4    Revision Date: 04/04/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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