FITC anti-human IgG Fc Antibody

Pricing & Availability
Clone
HP6017 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
HP6017_FITC_IgG_Antibody_FC_020813
Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) FITC (filled histogram) or mouse IgG2a, κ FITC isotype control (open histogram).
  • HP6017_FITC_IgG_Antibody_FC_020813
    Human DLL4 transfected CHO cells were incubated with recombinant human Notch 1-IgG Fc fusion protein, and then stained with anti-human IgG Fc (clone HP6017) FITC (filled histogram) or mouse IgG2a, κ FITC isotype control (open histogram).
See FITC spectral data
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This product has been discontinued, please use alternate products: Cat. Nos: 410719 and 410720 and Cat. No: 366915 and 366916.

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Description

IgG Fc is a homodimer composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Goat, Rabbit, Sheep, Horse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human immunoglobulin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HP6017 reacts with IgG (Fc fragment) of all subclasses. This antibody does not react with IgA, IgM, purified light chains, or the Fab fragment of IgG. This clone is not recommended for detection of transmembrane IgG on B cells. We recommend using clone M1310G05 as an alternative.

Application References

(PubMed link indicates BioLegend citation)
  1. Reime CB, et al. 1984. Hybridoma 3:263.
  2. Jefferis R, et al. 1985. Immunol. Lett. 10:223.
Product Citations
  1. Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
  2. Capello M, et al. 2019. Nat Commun. 10:254. PubMed
  3. Wen X, et al. 2019. Mol Biol Cell. 30:346. PubMed

Antigen Details

Structure
Homodimer formed by the constant region of the IgG heavy chain
Ligand/Receptor
CD16, CD32, CD64
Cell Type
B cells, Basophils, Dendritic cells, Macrophages, Mast cells, Neutrophils, NK cells, Platelets
Biology Area
Adaptive Immunity, Cell Biology, Immunology, Innate Immunity
Molecular Family
Fc Receptors
Antigen References

1. Paul WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia PA.

Gene ID
3500 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IgG Fc
Specificity Alt (DOES NOT SHOW ON TDS):
IgG Fc
UniProt
View information about IgG Fc on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 07/06/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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