FITC anti-human CD4 Antibody

Pricing & Availability
Clone
RPA-T4 (See other available formats)
Regulatory Status
RUO
Workshop
IV T114
Other Names
T4
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-T4_FITC_120108.jpg
Human peripheral blood lymphocytes stained with RPA-T4 FITC
  • RPA-T4_FITC_120108.jpg
    Human peripheral blood lymphocytes stained with RPA-T4 FITC
See FITC spectral data
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300505 25 tests $15.00
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300506 100 tests $45.00
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300538 500 tests $195.00
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, and blocking of T cell activation1,2.  This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Product Citations
  1. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  2. Goletz C, et al. 2018. Front Immunol. 9:1614. PubMed
  3. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  4. Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed
  5. Iio K, et al. 2019. Sci Rep. 9:813. PubMed
  6. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  7. Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed
  8. Hansen EC et al. 2016. eLife. 5 pii: e18447. PubMed
  9. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  10. Kang LJ, et al. 2020. Sci Rep. 10:5603. PubMed
  11. Mothe B, et al. 2020. Front Immunol. 1.029861111. PubMed
  12. Soldi R, et al. 2020. PLoS One. 15:e0235705. PubMed
  13. Handono K, et al. 2020. Eur J Dent. 0.961111111. PubMed
  14. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  15. Zenaro E, et al. 2009. J Leukoc Biol. 86:1393. PubMed
  16. Tamai Y, et al. 2013. J Immunol. 190:4382. PubMed
  17. Choudhry V, et al. 2006. Biochem Biophys Res Commun. 348:1107. PubMed
  18. Han L, et al. 2014. J Biol Chem. 289:25546. PubMed
  19. Luo X, et al. 2015. J Biol Chem. 290: 28675 - 28682. PubMed
  20. Wahl S, et al. 2016. Nature. 541:81-86. PubMed
  21. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
  22. Gong B, et al. 2021. Mol Med Rep. 23:00. PubMed
  23. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  24. Hamilton JR, et al. 2021. Cell Reports. 35(9):109207. PubMed
RRID
AB_314073 (BioLegend Cat. No. 300505)
AB_314074 (BioLegend Cat. No. 300506)
AB_2562052 (BioLegend Cat. No. 300538)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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