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FITC anti-human CD303 (BDCA-2) Antibody

Pricing & Availability
Clone
201A (See other available formats)
Regulatory Status
RUO
Other Names
CLEC4C, CLECSF11, CLECSF7, DLEC, HECL
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
201A_FITC_1_060512
Human peripheral blood mononuclear cells were stained with CD123 APC and CD303 (clone 201A) FITC (top) or mouse IgG2a, κ FITC isotype control (bottom). Data shown was gated on the lymphocyte and monocyte populations.
  • 201A_FITC_1_060512
    Human peripheral blood mononuclear cells were stained with CD123 APC and CD303 (clone 201A) FITC (top) or mouse IgG2a, κ FITC isotype control (bottom). Data shown was gated on the lymphocyte and monocyte populations.
  • 201A_FITC_2_060512
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354207 25 tests $118
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354208 100 tests $293
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Description

CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Freeman A, et al. 2014. PLoS One. 9:110928. PubMed
  2. Johnson P, et al. 2015. Clin Cnacer Res. 21:1321. PubMed
Product Citations
  1. Woo MS, et al. 2021. iScience. 24(7):102752. PubMed
  2. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  3. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  4. Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed
  5. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
  6. Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed
  7. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  8. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  9. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  10. Georgakis S, et al. 2021. JCI Insight. 6:. PubMed
  11. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  12. Singh A, et al. 2020. Cell Rep. 32:108153. PubMed
  13. Adhikaree J, et al. 2019. Oncoimmunology. 8:1593803. PubMed
  14. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  15. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  16. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
RRID
AB_2561363 (BioLegend Cat. No. 354207)
AB_2561364 (BioLegend Cat. No. 354208)

Antigen Details

Structure
Type II transmembrane protein, 38 kD, member of the C-type lectin superfamily
Distribution

Plasmacytoid dendritic cells

Function
Antigen capture, inhibition of IFN α/β production
Cell Type
Dendritic cells
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Jähn PS, et al. 2010. Cell Immunol. 265:15.
2. Graham LM and Brown GD. 2009. Cytokine 48:148.
3. Röck J, et al. 2007. Eur. J. Immunol. 37:3564.
4. Dzionek A, et al. 2001. J. Exp. Med. 194:1823.

Gene ID
170482 View all products for this Gene ID
UniProt
View information about CD303 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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