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Epitope Tag Big Motif Antibody Sampler Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Glutathione-S-transferase, Thioredoxin-1 (Trx1), Fip, FipA, TsnC, TrxA, Thioredoxin Tag, DSRED, Green fluorescent protein, GFP
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A_BigMotifSampler_anti-GST_WB_081817
15 µg of total protein from E. coli lysates with GST expression (Lane 1) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-GST antibody (clone P1A12). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Lane M: MW ladder.
  • A_BigMotifSampler_anti-GST_WB_081817
    15 µg of total protein from E. coli lysates with GST expression (Lane 1) were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-GST antibody (clone P1A12). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Lane M: MW ladder.
  • B_BigMotifSampler_anti-GFP_WB_081817
    15 µg each of total protein from 293E (lane2), 293E transfected with GFP fused protein (lane 3), were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-GFP antibody (clone 1GFP63). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control (lower). Lane M: MW ladder.
  • C_BigMotifSampler_anti-mCherry_WB_081817
    15 µg each of total protein from 293E (lane2), 293E transfected with mCherry fused protein (lane 4), were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti-mCherry antibody (clone 8C5.5). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control (lower). Lane M: MW ladder.
  • D_BigMotifSampler_anti-Thioredoxin1_WB_081817
    25 ng recombinant Thioredoxin-1 protein (Lane 5) was resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-Thioredoxin 1 antibody (clone 4H12A59). Proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Lane M: MW ladder.
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Description

The Epitope Tag Big Motif Antibody Sampler Kit provides a convenient resource to detect big Epitope Tag fused protein by Western Blot.

GST is commonly used as a tag to create fusion proteins in order to facilitate purification and detection of these proteins of interest. Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems. mCherry is a red fluorescent protein that is often used to tag target proteins and to monitor their subcellular localization. Thioredoxin is an 11.7 kD E. coli cytoplasmic protein. When used as a fusion partner to produce recombinant protein, thioredoxin has been shown to increase protein solubility and yield, as well as reduce protein aggregation and precipitation. 

For additional antigen details, please refer to individual product datasheets.

 

Product Details
Technical data sheet

Kit Contents

Kit Contents
Specificity Clone Size Reactivity Isotype MW (kD)
Anti-GST P1A12 25 µg GST tagged protein Mouse IgG2b, κ 26
Anti-GFP 1GFP63 25 µg GFP Mouse IgG2a 27
Anti-mCherry 8C5.5 25 µg mCherry Mouse IgG2a 27
Anti-Thioredoxin 1 4H12A59 25 µg E. coli Thioredoxin-1 and thioredoxin tagged recombinant protein Mouse IgG1, κ 12


* For detailed information about each specificity, please follow the provided links. 

Product Details

Formulation
Please refer to individual product datasheets for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Concentration
All antibodies in the kit are at 0.5mg/ml
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of antibodies in this kit is quality control tested by Western Blotting. For Western blotting, the suggested uses of these reagents are as follows:

Anti-GST: 0.05-0.5 µg/ml
Anti-GFP: 0.05-0.5 µg/ml
Anti-mCherry: 0.1-1 µg/ml
Anti-Thioredoxin 1: 0.05-0.5 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For verified or reported applications for these antibodies, please see individual product datasheets.

Application References

(PubMed link indicates BioLegend citation)
  1. Nicol C, et al. 2011. Biochem. Biophys. Res. Commun. 405:417. PubMed (Anti-GST)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
Go To Top Version: 1    Revision Date: 08/22/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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