- Poly4053 (See other available formats)
- Regulatory Status
- Goat Polyclonal IgG
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- Product Citations
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This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.Product Details
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography, and conjugated with DyLight™ 649 under optimal conditions.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* DyLight™ 649 has a maximum absorption of 655nm and a maximum emission of 670nm (similar to Alexa Fluor® 647 and APC).
- Application Notes
This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
(PubMed link indicates BioLegend citation)
- Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
- Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
- Winters T, et al. 2014. EMBO J. 33:1256-70.
- Product Citations
AB_1575128 (BioLegend Cat. No. 405312)
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