DAB Substrate Buffer with Stabilizer (Previously Covance catalog# SIG-31049)

Pricing & Availability
Regulatory Status
Other Names
Chromogen Substrate Solution
Covance Catalog# SIG-31049
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926901 2 x 50 mL $65.00
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Technical data sheet

Product Details

Chromogen Substrate Buffer.
Storage & Handling
Store between 2°C and 8°C.


Recommended Usage

1. Add the DAB chromogen to the DAB substrate buffer as described in the table below

Buffer     DAB Chromogen*
5 mL   +   0.2 mL
25 mL   +   1.0 mL
50 mL   +   2.0 mL
*Cat Nos: 926501 & 926503.

2. Apply mixture to tissue section 
3. Incubate the slides for 5-10 min. at room temperature
4. Rinse tissue sections with deionized water
5. Counterstain and mount
6. DAB produces a brown precipitate with almost no background

Application Notes

BioLegend’s DAB Substrate Buffer with Stabilizer consists of stable buffer. The DAB Substrate Buffer with Stabilizer in combination with DAB chromogen reacts with peroxidase to produce brown staining.

Antigen Details

Gene ID

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Go To Top Version: 2    Revision Date: 07/17/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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