- B3B4 (See other available formats)
- Regulatory Status
- Other Names
- FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD23 is a 45 kD protein also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion.Product Details
- Antibody Type
- Host Species
- Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, and immunohistochemical staining of acetone-fixed frozen sections5.
(PubMed link indicates BioLegend citation)
- Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
- Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
- Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
- Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
- Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
- Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed
AB_2832279 (BioLegend Cat. No. 101637)
- C-lectin family, type II transmembrane glycoprotein, 45 kD
B cells, follicular dendritic cells
- Regulates B cell activation
- IgE, CD19/CD21/CD81
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.
- Gene ID
- 14128 View all products for this Gene ID
- View information about CD23 on UniProt.org
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