Brilliant Violet 605™ anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_BV605_051812
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Brilliant Violet 605™ (filled histogram) or rat IgG2a, κ Brilliant Violet 605™ isotype control (open histogram).
  • 6D5_BV605_051812
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Brilliant Violet 605™ (filled histogram) or rat IgG2a, κ Brilliant Violet 605™ isotype control (open histogram).
See Brilliant Violet 605™ spectral data
Cat # Size Price Quantity Check Availability Save
115539 125 µL $190.00
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115540 50 µg $260.00
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/ml
µl sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  2. Marcus A et al. 2018. Immunity. 49(4):754-763 . PubMed
  3. Van Winkle JA et al. 2018. Cell host & microbe. 24(5):665-676 . PubMed
  4. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  5. Yu‐Han Chang et al. 2017. Immunity. 47(5):943-958 . PubMed
  6. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  7. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  8. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  9. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  10. John S, et al. 2020. Sci Rep. 10:11377. PubMed
  11. Pokrovskii M, et al. 2019. Immunity. 51:185. PubMed
  12. Li J, et al. 2018. Immunity. 49:178. PubMed
  13. Jamali A, et al. 2020. Cell Reports. 32(9):108099. PubMed
  14. Benhamou D, et al. 2018. Front Immunol. 2.260416667. PubMed
  15. Colliou N, et al. 2015. Toxins. 7: 3805 - 3817. PubMed
  16. Bramwell K, et al. 2015. J Immunol. 195: 1647 - 1656. PubMed
  17. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  18. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  19. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  20. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  21. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
  22. White JM, et al. 2020. Mol Pharm. 2.165972222. PubMed
  23. Rosenkranz SC, et al. 2021. eLife. 10:00. PubMed
  24. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  25. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  26. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  27. Maisonneuve C, et al. 2021. Cell Reports. 34(4):108677. PubMed
  28. Earnest JT, et al. 2021. Cell Reports. 35(1):108962. PubMed
  29. Nascimento DC, et al. 2021. Immunity. :. PubMed
  30. Hutter K, et al. 2020. FEBS J. . PubMed
RRID
AB_11203538 (BioLegend Cat. No. 115539)
AB_2563067 (BioLegend Cat. No. 115540)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/24/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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