Brilliant Violet 605™ anti-human CD103 (Integrin αE) Antibody

Pricing & Availability
Clone
Ber-ACT8 (See other available formats)
Regulatory Status
RUO
Workshop
V A067
Other Names
Integrin alpha E (ITGAE)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Ber-ACT8_BV605_CD103_Antibody_FC_1_091914
PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD3 APC/Cy7 and CD103 (clone Ber-ACT8) Brilliant Violet 605™ (top) or mouse IgG1, κ Brilliant Violet 605™ isotype control (bottom).
  • Ber-ACT8_BV605_CD103_Antibody_FC_1_091914
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD3 APC/Cy7 and CD103 (clone Ber-ACT8) Brilliant Violet 605™ (top) or mouse IgG1, κ Brilliant Violet 605™ isotype control (bottom).
  • Ber-ACT8_BV605_CD103_Antibody_FC_2_091914
See Brilliant Violet 605™ spectral data
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350217 25 tests $185.00
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350218 100 tests $390.00
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Description

CD103 is a type I transmembrane glycoprotein also known as αE integrin, integrin αIEL chain, and human mucosal lymphocyte antigen 1. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. Treg cells express high level of CD103. Hairy cell leukemia has also been shown to express CD103. The expression of CD103 on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as an αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HTLV-1 induced human T cell line MAPS16
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western Blotting1, immunoprecipitation1, and immunohistochemical staining of frozen tissue sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636. (WB, IP, IHC-F)
  2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  2. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  3. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  4. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  5. Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
  6. Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
  7. Singh A, et al. 2020. Cell Rep. 32:108153. PubMed
  8. Luoma AM, et al. 2020. Cell. 182(3):655-671.e22. PubMed
  9. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
RRID
AB_2564282 (BioLegend Cat. No. 350217)
AB_2564283 (BioLegend Cat. No. 350218)

Antigen Details

Structure
Type I transmembrane glycoprotein, integrin family; can be cleaved into 150 kD and 25 kD chains; associated with β7 integrin
Distribution

Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells; expressed on hairy cell leukemia

Function
Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulation of tissue-specific T cell homing
Ligand/Receptor
E-Cadherin
Cell Targets
Integrin β7
Cell Type
Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Parker CM, et al. 1992. P. Natl. Acad. Sci. USA 89:1924.
2. Kruschwitz M, et al. 1991. J. Clin. Pathol. 44:636.
3. Schon MP, et al. 1999. J. Immunol. 162:6641.
4. Shaw SK, et al. 1994. J. Biol. Chem. 269:6016.

Gene ID
3682 View all products for this Gene ID
UniProt
View information about CD103 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/24/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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