Brilliant Violet 570™ anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
HCD56 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HCD56_BV570_1_072611
Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
  • HCD56_BV570_1_072611
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
  • HCD56_BV570_2_072611
See Brilliant Violet 570™ spectral data
Cat # Size Price Quantity Avail. Save
318329 25 tests $225.00
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318330 100 tests $405.00
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Chirkova T, et al. 2013. J Virol. 87:13466. PubMed
RRID
AB_10895920 (BioLegend Cat. No. 318329)
AB_2563837 (BioLegend Cat. No. 318330)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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