- 14G2a (See other available formats)
- Regulatory Status
- Other Names
- Disialoganglioside GD2, Ganglioside G2
- Mouse IgG2a, κ
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- Product Citations
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Ganglioside GD2 is a sialic acid-containing glycosphingolipid involved in cell attachment to the extracellular matrix. Expression of GD2 in normal tissue is restricted to cells from the central nervous system, peripheral nerves, skin melanocytes, and mesenchymal stem cells. However GD2 is highly expressed by tumors of neuro-ectodermal origin such as melanomas, gliomas, neuroblastomas, and small cell lung carcinoma. GD2 has been proposed as a marker for some cancer stem cells.Product Details
- Antibody Type
- Host Species
- Neuroblastoma cell line LAN-1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Clone 14G2a is an isotype switch variant from parental hybridoma 14.18 (IgG3)1. Additional reported applications (for the relevant formats) include: inducing apoptosis and enhancing cytotoxicity of chemotherapeutic drugs in the neuroblastoma cell line 2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for use in cytotoxic and apoptotic studies (contact our custom solutions team). This clone has also been published as 14.G2a.
(PubMed link indicates BioLegend citation)
- Mujoo K, et al. 1989. Cancer Res. 49:2857. (Cyt)
- Kowalczyk A, et al. 2009. Cancer Lett. 281:171. (Apop, Cyt)
- Battula VL, et al. 2012. J. Clin. Invest. 122:2066. (FC)
AB_2800978 (BioLegend Cat. No. 357315)
AB_2800979 (BioLegend Cat. No. 357316)
Neurons, mesenchymal stem cells, highly expressed in tumors of neuro-ectodermal origin, such as melanomas, gliomas, neuroblastomas, and small cell lung carcinoma
- Cell attachment to extracellular matrix
- Cell Type
- Mesenchymal cells, Neurons
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Immunology, Neuroscience
- Molecular Family
- Adhesion Molecules
- Antigen References
1. Tarek N, et al. 2012. J. Clin. Invest. 122:3260.
2. Matthay KK, et al. 2012. Clin. Cancer Res. 18:2740.
3. Navid F, et al. 2010. Curr. Cancer Drug Targets. 10:200.
- Gene ID
- 2583 View all products for this Gene ID
- View information about Ganglioside GD2 on UniProt.org
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