Brilliant Violet 421™ anti-mouse CD31 Antibody

Pricing & Availability
Clone
390 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
390_BV421_CD31_Antibody_FC_061013
C57BL/6 mouse splenocytes were stained with CD31 (clone 390) Brilliant Violet 421™ (open histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • 390_BV421_CD31_Antibody_FC_061013
    C57BL/6 mouse splenocytes were stained with CD31 (clone 390) Brilliant Violet 421™ (open histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
102423 125 µL $165.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
102424 50 µg $260.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µl size: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
µg size: 0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed
  2. Naito H, et al. 2019. Dev Cell. 48:151. PubMed
  3. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  4. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  5. Dong W, et al. 2020. Sci Rep. 10:6652. PubMed
  6. Romera–Hernández M, et al. 2020. Cell Reports. 30(1):37-45.e3.. PubMed
  7. Kugler J, et al. 2015. Toxicol Sci. 146: 374-385. PubMed
  8. Dokun A, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 790-803. PubMed
  9. Kang MH, et al. 2020. Nat Commun. 3.186805556. PubMed
  10. Huetter J, et al. 2020. Cancer Immunol Res. 0.954861111. PubMed
  11. Hu B, et al. 2020. J Clin Invest. 130:3483. PubMed
  12. Naito H, et al. 2020. Nature Protocols. 15(3):1066-1081. PubMed
  13. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  14. Redpath AN, et al. 2021. STAR Protocols. 2(1):100359. PubMed
  15. Frii J, et al. 2021. Immunity. 54(5):1002-1021.e10. PubMed
  16. Chuang CH, et al. 2020. Cancer Research. 81(3):567-579. PubMed
  17. Quaife-Ryan GA, et al. 2020. Development. 147: . PubMed
RRID
AB_2562186 (BioLegend Cat. No. 102423)
AB_2650892 (BioLegend Cat. No. 102424)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 0    Revision Date: 03/29/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account