Brilliant Violet 421™ anti-mouse CD185 (CXCR5) Antibody

Pricing & Availability
Clone
L138D7 (See other available formats)
Regulatory Status
RUO
Other Names
BLR1, GPCR6, MDR-15
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
L138D7_BV421_CXCR5_Antibody_FC_1_052213
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CXCR5 (clone L138D7, top) Brilliant Violet 421™ or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • L138D7_BV421_CXCR5_Antibody_FC_1_052213
    C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CXCR5 (clone L138D7, top) Brilliant Violet 421™ or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
  • L138D7_BV421_CXCR5_Antibody_FC_2_052213
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
145511 125 µL $170.00
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145512 500 µL $375.00
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Description

CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mCXCR5-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.

Product Citations
  1. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  2. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  3. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  4. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  5. Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed
  6. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  7. Dheilly E, et al. 2020. Cancer Cell. 37(5):674-689. PubMed
  8. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  9. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  10. Wrzner K, et al. 2021. EBioMedicine. 63:103197. PubMed
  11. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  12. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  13. Sato R, et al. 2020. Int Immunol. 499:32. PubMed
RRID
AB_2562127 (BioLegend Cat. No. 145511)
AB_2562128 (BioLegend Cat. No. 145512)

Antigen Details

Structure
42 kD, seven transmembrane protein
Distribution

B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain

Function
B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
Ligand/Receptor
BLC/CXCL13
Cell Type
B cells, T cells, Tfh
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532.
2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381.
3. Forster R, et al. 1994. Blood 84:830.
4. Forster R, et al. 1996. Cell 87:1037.
5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.

Gene ID
12145 View all products for this Gene ID
UniProt
View information about CD185 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 06/05/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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