Brilliant Violet 421™ anti-human GARP (LRRC32) Antibody

Pricing & Availability
Clone
7B11 (See other available formats)
Regulatory Status
RUO
Other Names
Garpin, Glycoprotein A repetitions predominant
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
7B11_BV421_GARP_Antibody_FC_1_062613
Human peripheral mononuclear blood cells were stimulated with CD3, CD28 and recombinant human IL-2 for 24 hours and then stained with CD4 PerCP, LAP (TGF-β) APC and GARP (clone 7B11) Brilliant Violet 421™ (top) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom). Data was analyzed by gated on CD4-positive cells.
  • 7B11_BV421_GARP_Antibody_FC_1_062613
    Human peripheral mononuclear blood cells were stimulated with CD3, CD28 and recombinant human IL-2 for 24 hours and then stained with CD4 PerCP, LAP (TGF-β) APC and GARP (clone 7B11) Brilliant Violet 421™ (top) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (bottom). Data was analyzed by gated on CD4-positive cells.
  • 7B11_BV421_GARP_Antibody_FC_2_062613
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
352509 25 tests $195.00
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Description

Glycoprotein A Repetitions Predominant (GARP), also known as leucine rich repeat containing 32 (LRC32), is a 80 kD type I membrane glycoprotein with 20 leucine rich repeats in the extracellular portion of the protein. GARP was found on the surface of megakaryocytes, platelets, and activated Tregs (CD4+, CD25+, FoxP3+ cells) and serves as a receptor for latent TGF-β. Recent evidence suggests that GARP may play a role in controlling suppressor function of Tregs. A mutation in GARP has been reported in a large Samaritan kindred with Usher syndrome type 1, an autosomal recessive disease characterized by profound congenital sensorineural deafness, vestibular dysfunction, and progressive visual loss. In addition, it has been found that GARP mRNA is highly amplified in different tumors, which indicates that tumor cells may use GARP to express TGF-β or to capture TGF-β from their surroundings, resulting in local suppression of anti-tumor immune responses or the induction of Tregs.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
LRRC32-DNA vaccination
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
RRID
AB_2562249 (BioLegend Cat. No. 352509)

Antigen Details

Structure
80 kD transmembrane GARP glycoprotein with an extracellular region containing 20 leucine-rich repeats
Distribution

Activated Tregs, megakaryocytes, platelets

Ligand/Receptor
LAP (TGF-β1)
Cell Type
Megakaryocytes, Platelets, Tregs
Biology Area
Cell Biology, Immunology, Signal Transduction
Antigen References

1. Ollendorff V, et al. 1994. Cell. Growth Differ. 5:213.
2. Stockis J, et al. 2009. Eur. J. Immunol. 39:3315.
3. Wang R, et al. 2009. P. Natl. Acad. Sci. USA 106:13439.
4. Tran DQ, et al. 2009. P. Natl. Acad. Sci. USA 106:13445.

Gene ID
2615 View all products for this Gene ID
UniProt
View information about GARP on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 06/25/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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