Brilliant Violet 421™ anti-human CD3 Antibody

Pricing & Availability
Clone
SK7 (See other available formats)
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SK7_BV421_CD3_FC_antibody_043015.jpg
Human peripheral blood lymphocytes were stained with CD3 (clone SK7) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • SK7_BV421_CD3_FC_antibody_043015.jpg
    Human peripheral blood lymphocytes were stained with CD3 (clone SK7) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
344833 25 tests $160
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344834 100 tests $345
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.

Application References

(PubMed link indicates BioLegend citation)
  1. Kan EA, et al. 1983. J. Immunol. 131:536.
  2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
  3. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  4. Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
  5. Carbone A, et al. 1999. Blood 93:2319. (IHC)
  6. Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
  7. Rutjens E, et al. 2007. J. Immunol. 178:1702.
  8. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
  2. Kosti P, et al. 2021. Cell Reports Medicine. 2(4):100227. PubMed
RRID
AB_2565674 (BioLegend Cat. No. 344833)
AB_2565675 (BioLegend Cat. No. 344834)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, during thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 04/30/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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