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Brilliant Violet 421™ anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_BV421_1_032911
Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • HIB19_BV421_1_032911
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • HIB19_BV421_2_032911
  • HIB19_BV421_CD19_Antibody_2_012914
    Human peripheral mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/mL CD19 (clone HIB19) Brilliant Violet 421™ (blue) and 5 µg/mL CD3 (clone UCHT1) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.
  • HIB19_BV421_CD19_Antibody_3_012914
    Human frozen tonsil tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 5 µg/mL of Alexa Fluor® 488 anti-human CD3 antibody (green) and Brilliant Violet 421™ anti-human CD19 antibody (blue) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
Compare all formats See Brilliant Violet 421™ spectral data
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302233 25 tests $190
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302234 100 tests $381
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  2. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  3. De Hert E, et al. 2022. Int J Mol Sci. 23:. PubMed
  4. Nakano M, et al. 2021. Front Immunol. 12:713225. PubMed
  5. Pholcharee T, et al. 2021. Nat Commun. 12:1063. PubMed
  6. Panwar B, et al. 2021. Genome Res. 31:659. PubMed
  7. Turner JS, et al. 2021. Nature. 596:109. PubMed
  8. Sun Q, et al. 2022. Cell Death Dis. 13:724. PubMed
  9. O’Connor K, et al. 2016. Genes Immun. 10.1038/gene.2016.27. PubMed
  10. Mimitou EP, et al. 2021. Nat Biotechnol. 39:1246. PubMed
  11. Vijayakumar B, et al. 2022. Immunity. . PubMed
  12. Travaglini KJ, et al. 2020. Nature. 587:619. PubMed
  13. Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
  14. Wouters K, et al. 2017. Sci Rep. 7:42665. PubMed
  15. Koppejan H, et al. 2019. Arthritis Res Ther. 21:03. PubMed
  16. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  17. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  18. Singh M, et al. 2020. Cell. 180(5):878-894. PubMed
  19. Caeser R, et al. 2021. Nat Protoc. 16:2499. PubMed
  20. Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed
  21. Zumaquero E, et al. 2019. Elife. 8. PubMed
  22. Shih T, et al. 2019. Front Immunol. 10:1652. PubMed
  23. NULL, et al. 2020. J Clin Immunol. 40:1. PubMed
  24. De Silva TI, et al. 2021. iScience. 24:103353. PubMed
  25. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  26. Tan Y, et al. 2014. Cell Immunol. 151:55. PubMed
  27. Schoofs T et al. 2019. Immunity. 50(6):1513-1529 . PubMed
  28. Usmani SM et al. 2019. Cell host & microbe. 25(1):73-86 . PubMed
  29. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
  30. Ju CH, et al. 2018. Clin Immunol. 193:70. PubMed
  31. Antonarakis ES, et al. 2018. Clin Cancer Res. 24:4662. PubMed
  32. Verboven K, et al. 2018. Sci Rep. 8:4677. PubMed
  33. Turner JS, et al. 2020. Nature. 586:127. PubMed
  34. Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
  35. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  36. Zheng H, et al. 2022. Cell Rep. 39:110864. PubMed
  37. Amara K, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.03.004. PubMed
  38. Lu D, et al. 2014. Cell Immunol. 152:77. PubMed
  39. Baguma R, et al. 2017. PLoS One. 10.1371/journal.pone.0184563. PubMed
  40. Tian P, et al. 2022. Front Cell Dev Biol. 10:835321. PubMed
RRID
AB_10897802 (BioLegend Cat. No. 302233)
AB_11142678 (BioLegend Cat. No. 302234)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD19 HIB19 FC
Biotin anti-human CD19 HIB19 FC
FITC anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
Purified anti-human CD19 HIB19 FC,CyTOF®,IHC-F
APC/Cyanine7 anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
Alexa Fluor® 488 anti-human CD19 HIB19 FC,ICC
Alexa Fluor® 647 anti-human CD19 HIB19 FC,IHC-F
Pacific Blue™ anti-human CD19 HIB19 FC
Alexa Fluor® 700 anti-human CD19 HIB19 FC
PerCP anti-human CD19 HIB19 FC
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Brilliant Violet 421™ anti-human CD19 HIB19 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD19 HIB19 FC
Brilliant Violet 650™ anti-human CD19 HIB19 FC
Brilliant Violet 785™ anti-human CD19 HIB19 FC
Brilliant Violet 510™ anti-human CD19 HIB19 FC
Brilliant Violet 605™ anti-human CD19 HIB19 FC
Brilliant Violet 711™ anti-human CD19 HIB19 FC
Purified anti-human CD19 (Maxpar® Ready) HIB19 FC,CyTOF®
Alexa Fluor® 594 anti-human CD19 HIB19 ICC,IHC-F
PE/Dazzle™ 594 anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
Pacific Blue™ anti-human CD19 HIB19 FC
APC anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
TotalSeq™-A0050 anti-human CD19 HIB19 PG
Brilliant Violet 750™ anti-human CD19 HIB19 FC
TotalSeq™-B0050 anti-human CD19 HIB19 PG
TotalSeq™-C0050 anti-human CD19 HIB19 PG
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Spark NIR™ 685 anti-human CD19 HIB19 FC
Ultra-LEAF™ Purified anti-human CD19 HIB19 FC,CyTOF®,Block,IHC
APC/Fire™ 810 anti-human CD19 HIB19 FC
PE/Fire™ 640 anti-human CD19 HIB19 FC
PE/Fire™ 700 anti-human CD19 HIB19 FC
TotalSeq™-D0050 anti-human CD19 HIB19 PG
Spark YG™ 593 anti-human CD19 HIB19 FC
GMP Pacific Blue™ anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
GMP PE anti-human CD19 HIB19 FC
GMP APC anti-human CD19 HIB19 FC
KIRAVIA Blue 520™ anti-human CD19 HIB19 FC
GMP PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
GMP PE/Cyanine7 anti-human CD19 HIB19 FC
Spark Violet™ 500 anti-human CD19 HIB19 FC
PE/Fire™ 810 anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
PerCP/Fire™ 806 anti-human CD19 HIB19 FC
PerCP/Fire™ 780 anti-human CD19 HIB19 FC
Spark PLUS UV™ 395 anti-human CD19 HIB19 FC
Spark Red™ 718 anti-human CD19 HIB19 FC
Go To Top Version: 4    Revision Date: 03/19/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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