Brilliant Violet 421™ anti-human CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A019D5 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A019D5_BV421_1_040311
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • A019D5_BV421_1_040311
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • A019D5_BV421_2_040311
See Brilliant Violet 421™ spectral data
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351309 25 tests $180.00
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351310 100 tests $365.00
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD127
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported (for the relevant formats) application: proteogenomics1.

Additional Product Notes

View more applications data using this product to resolve dim populations, determine staining index, and to detect CLPs in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Trabanelli S, et al. 2017. Nat Commun. 10.1038/s41467-017-00678-2. PubMed
  2. Wright AKA, et al. 2017. J Allergy Clin Immunol. 140:1430. PubMed
  3. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
  4. Cuadrado E, et al. 2018. Immunity. 48:1046. PubMed
  5. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  6. de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed
  7. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
  8. Zou F, et al. 2019. Nat Commun. 10:4109. PubMed
  9. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  10. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  11. Judge CJ, et al. 2017. J Leukoc Biol. 102:171. PubMed
  12. Burke MT, et al. 2017. Transplant Direct. 3:e171. PubMed
  13. Lev A, et al. 2019. Front Immunol. 1.577777778. PubMed
  14. Pasciuto E, et al. 2020. Cell. 182:625. PubMed
  15. Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
  16. Xu Y, et al. 2013. J Virol. 87:3760. PubMed
  17. Xu Y, et al. 2013. J Immunol Methods. 394:73. PubMed
  18. Willmann K, et al. 2014. Nat Commun. 5:5360. PubMed
  19. Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed
  20. Causi E, et al. 2015. PLoS One. 10: 0136717. PubMed
  21. Grove K, et al. 2016. PLoS One. 11: 0145961. PubMed
  22. Kimura K, et al. 2016. Neurol Neuroimmunol Neuroinflamm. 3: e210. PubMed
  23. Eller MA, et al. 2016. J Virol. 90: 4005-4016. PubMed
  24. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  25. Kim DH, et al. 2021. eLife. 10:00. PubMed
  26. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  27. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
  28. Crump NT, et al. 2021. Cell Reports. 35(6):109101. PubMed
  29. Kasatskaya SA, et al. 2020. Elife. :9. PubMed
RRID
AB_10898326 (BioLegend Cat. No. 351309)
AB_10960140 (BioLegend Cat. No. 351310)

Antigen Details

Structure
Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

Gene ID
3575 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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