Brilliant Violet 421™ anti-human CD107b (LAMP-2) Antibody

Pricing & Availability
Clone
H4B4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
LAMPb, LGP110
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_H4B4_BV421_CD107b_Antibody_013019_updated.png
Human acute myeloid leukemia cell line KG1a was fixed, permeabilized, then intracellularly stained with CD107b (LAMP-2) (clone H4B4) Brilliant Violet 421™ (filled histogram) or Mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • 1_H4B4_BV421_CD107b_Antibody_013019_updated.png
    Human acute myeloid leukemia cell line KG1a was fixed, permeabilized, then intracellularly stained with CD107b (LAMP-2) (clone H4B4) Brilliant Violet 421™ (filled histogram) or Mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • 2_H4B4_BV421_CD107b_Antibody_IHC_111317
    Human paraffin‐embedded prostate tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris‐Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with anti-human CD107b (clone H4B4) Brilliant Violet 421™ (blue) and anti-human CD44 (clone IM7) Alexa Fluor® 594 (red) overnight at 4°C. The nuclei were counterstained with DRAQ5™ (green). The image was scanned with a 10X objective and stitched with MetaMorph® software.
See Brilliant Violet 421™ spectral data
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354307 25 µg $165.00
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354308 100 µg $355.00
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Description

CD107b, also known as LAMP-2, is a 105 kD, highly gylcosylated, type I transmembrane protein. CD107b is expressed in lysosomal/endosomal membranes in nearly all cells, and on the surface of activated platelets, activated lymphocytes and some tumor cell lines.  LAMP-2 is known to have roles in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Adult human adherent spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.4 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 2 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen glomeruli2 and immunofluorescent staining of  neutrophils2,3.

This antibody is specific to human LAMP-2. Positive control: Hela cells; LAMP-2 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References

(PubMed link indicates BioLegend citation)
  1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
  2. Kain R, et al. 2008. Nat. Med. 14:1088. (IF, IHC)
  3. Roark EA, et al. 2008. PLoS ONE 3:e3538. (IF)
RRID
AB_2721512 (BioLegend Cat. No. 354307)
AB_2721513 (BioLegend Cat. No. 354308)

Antigen Details

Structure
LAMP-2 is a 410 amino acid protein with a molecular mass of 45 kD.
Distribution

Lysosomal/endosomal membranes in nearly all cells; surface of activated platelets, activated lymphocytes and some tumor cell lines

Function
Adhesion, cellular homeostatis, including autophagocytosis, antigen presentation
Cell Type
B cells, Lymphocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
2. Kain R, et al. 2008. Nat. Med. 14:1088.
3. Roark EA, et al. 2008. PLoS ONE 3:e3538.

Gene ID
3920 View all products for this Gene ID
UniProt
View information about CD107b on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 01/30/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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