- OX-1 (See other available formats)
- Other Names
- Leukocyte common antigen, LCA, T200, Ly-5
- Mouse IgG1, κ
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- Product Citations
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CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro.Product Details
- Antibody Type
- Host Species
- Enriched glycoprotein fraction from Wistar rat thymocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy (acetone fixed, ammonium-thiocyanate separated epidermal sheets)2, immunohistochemistry of acetone3-, isopentane-4, PLP5-, and n-hexane6 fixed frozen sections and paraffin-embedded sections7,8, and partial inhibition of NK cell lysis of syngeneic tumor cell lines1.
(PubMed link indicates BioLegend citation)
- Giezeman-Smits KM, et al. 1999. J. Immunol. 163:71. (IP)
- Elbe A, et al. 1994. J. Invest. Dermatol. 102:74. (IF)
- Kouwenhoven E, et al. 2001. Kidney Int. 59:1142. (IHC)
- Martin A, et al. 1995 Clin. Exp. Immunol. 22:283. (IHC)
- Sayegh MH, et al. 1995 J. Exp. Med. 181:186. (IHC)
- Morioka Y, et al. 2000. Kidney Int. 60:2192. (IHC)
- Ng YY, et al. 2005. Kidney Int. 94:S83. (IHC-P)
- Huang XR, et al. 2003. JASN. 14:1738. (IHC-P)
- Product Citations
AB_314004 (BioLegend Cat. No. 202203)
- Protein tyrosine phosphatase, type I transmembrane protein, 180-220 kD (multiple isoforms and differential glycosylation)
All hematopoietic cells except erythrocytes and platelets
- T cell receptor and B cell receptor signal transduction
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol.. 15:168.
- Gene ID
- 24699 View all products for this Gene ID
- View information about CD45 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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