Biotin anti-mouse IL-34 Antibody

Pricing & Availability
Clone
Poly5193 (See other available formats)
Regulatory Status
RUO
Other Names
IL34, Interleukin 34, C16orf77
Isotype
Goat Polyclonal Ig
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
519303 50 µg $225.00
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Description

IL-34 is a homodimeric glycoprotein which contains 241 amino acids. IL-34 is expressed in the heart, brain, lung, liver, kidney, thymus, spleen, and other organs. It specifically binds to CD115/M-CSF receptor (CSF-1 receptor or c-Fms) and activates ERK1/2 phosphorylation. The function of IL-34 overlaps with CSF-1 to regulate myeloid lineage differentiation, proliferation, and survival.

Elevated levels of IL-34 are seen in the plasma of rheumatoid arthritis patients, but can be decreased in response to therapy. IL-34 levels are also increased in the serum of cancer patients and associated with poor prognosis.

Mouse IL-34 shares 71% sequence identity with human IL-34 on the amino acid level.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant mouse IL-34
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 0.5 µg/ml detection antibody was utilized to generate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application Notes

ELISA Detection: To measure mouse IL-34, this antibody can be used as a detection antibody in sandwich ELISA format, and paired with the LEAF™ purified C054-35 antibody (Cat. No. 147201) as the capture antibody. Recombinant Mouse IL-34 (ELISA Std.) (Cat. No. 577609) can be used as the protein standard.

Note: For testing mouse IL-34 in serum, plasma or cell culture supernatant, BioLegend's LEGEND MAX™ ELISA Kits (Cat. No. 439107 & 439108) are specially developed and recommended.

RRID
AB_2561419 (BioLegend Cat. No. 519303)

Antigen Details

Structure
Homodimer
Distribution

Expressed in different tissues, including spleen, heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostate, and colon

Function
Regulates myeloid lineage differentiation, proliferation, and survival
Interaction
Macrophages, monocytes, and myeloid cells
Ligand/Receptor
CD115/M-CSF receptor (CSF-1 receptor or c-Fms)
Cell Type
Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lin H, et al. 2008. Sciences 320:807.
2. Chihara T, et al. 2010. Cell Death Differ. 17:1917.
3. Wei S, et al. 2010. J. Leuko. Biol. 88:495.
4. Eda H, et al. 2011. Rheumatol 31:1525.
5. Covaleda L, et al. 2010. Virology 397:217.

Gene ID
76527 View all products for this Gene ID
UniProt
View information about IL-34 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 02/06/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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