Biotin anti-mouse IL-25 (IL-17E) Antibody

Pricing & Availability
M3704D11 (See other available formats)
Regulatory Status
Other Names
Interleukin 25, Interleukin 17E
Rat IgG2a, κ
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538104 100 µg $225.00
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IL-25 (also known as IL-17E) is a member of the IL-17 family, which includes IL-17A, IL-17B, IL-17C, IL-17F, and IL-17A/F. The primary sequence homology differs between family members, with mouse IL-17A and IL-17F having the highest homology (49%), while mouse IL-17A and IL-25 have the lowest (28%). IL-25 induces gene expression of IL-4, IL-5, and IL-13 in multiple tissues, and this induction results in T helper 2 (TH2)-type immune responses; this is characterized by an increase in serum IgE levels, pathologic changes in the lungs and digestive tract with eosinophilic infiltrates, an increase in mucus production, epithelial cell hyperplasia, and overall amplified allergic inflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Recombinant Mouse IL-25
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 1 - 8 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-25 (IL-17E) recombinant protein ranging from 1600 to 25 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The biotinylated M3704D11 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the pure M3706F07 antibody (Cat. No. 676304) as the capture antibody and Recombinant Recombinant Mouse IL-25 (IL-17E) (ELISA Std.) (Cat. No. 579809).

AB_2814449 (BioLegend Cat. No. 538104)

Antigen Details


IL-25 is produced by mast cells, epithelial cells, alveolar macrophages, microglia, and Th2 cells

IL-25 directly promotes Th2 cell differentiation and the expression of IL-4, IL-5, IL-13, CCL11 (eotaxin), and therefore, lung eosinophilia. IL-25 enhances IL-9 production by differentiated Th9 cells
CD4+ NKT cells, macrophages, endothelial cells, eosinophils, and Th2 and Th9 cells
IL-25 binds to IL-17RB
Antigen References
  1. Hurst SD, et al. 2002. J. Immunol. 169:443.
  2. Ikeda K, et al. 2003. Blood 101:3594.
  3. Kang CM, et al. 2005. Am. J. Respir. Cell. Mol. Biol. 33:290.
  4. Kleinschek ME, et al. 2007. J. Exp. Med. 204:161.
  5. Terashima A, et al. 2008. J. Exp. Med. 205:2727.
  6. Angkasekwinai P, et al. 2010. Nat. Immunol. 11:250
Gene ID
140806 View all products for this Gene ID
View information about IL-25 on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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