Biotin anti-mouse CD335 (NKp46) Antibody

Pricing & Availability
Clone
29A1.4 (See other available formats)
Regulatory Status
RUO
Other Names
NKp46, NCR1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
29A1.4_Biotin_1_060412
C57/BL6 mouse splenocytes were stained with NK1.1 Brilliant Violet 421™ and biotinylated anti- mouse CD335 (clone 29A1.4, top) or rat IgG2a, κ isotype control (bottom), followed by Sav-PE.
  • 29A1.4_Biotin_1_060412
    C57/BL6 mouse splenocytes were stained with NK1.1 Brilliant Violet 421™ and biotinylated anti- mouse CD335 (clone 29A1.4, top) or rat IgG2a, κ isotype control (bottom), followed by Sav-PE.
  • 29A1.4_Biotin_2_060412
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137615 50 µg $80.00
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137616 200 µg $220.00
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Description

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
NKP46-IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.

Application References

(PubMed link indicates BioLegend citation)
  1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
  2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
  3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
Product Citations
  1. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  2. Shirley SA, et al. 2020. Cancers (Basel). 12:00. PubMed
RRID
AB_11219387 (BioLegend Cat. No. 137615)
AB_11218796 (BioLegend Cat. No. 137616)

Antigen Details

Structure
Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution

Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells

Function
NK cells activation
Ligand/Receptor
Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.

Gene ID
17086 View all products for this Gene ID
UniProt
View information about CD335 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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