- C9B7W (See other available formats)
- Regulatory Status
- Other Names
- LAG3, LAG-3
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.Product Details
- Antibody Type
- Host Species
- Murine CD223-Ig fusion protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
(PubMed link indicates BioLegend citation)
- Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
- Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
- Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
- Product Citations
AB_961177 (BioLegend Cat. No. 125205)
AB_2133345 (BioLegend Cat. No. 125206)
- Ig superfamily
T cells and NK cells, as well as T regulatory cells
- Cell Type
- NK cells, T cells, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Baixeras E et al. 1992. J. Exp. Med. 176:327.
- Gene ID
- 16768 View all products for this Gene ID
- View information about CD223 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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