Biotin anti-mouse CD2 Antibody

Pricing & Availability
Clone
RM2-5 (See other available formats)
Regulatory Status
RUO
Other Names
LFA-2, T11, Ly-37, SRBC-R
Isotype
Rat IgG2b, λ
Ave. Rating
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Product Citations
publications
RM2-5_Biotin_021306
C57BL/6 mouse splenocytes stained with biotinylated RM2-5, followed by Sav-PE
  • RM2-5_Biotin_021306
    C57BL/6 mouse splenocytes stained with biotinylated RM2-5, followed by Sav-PE
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100103 50 µg $65.00
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100104 500 µg $225.00
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Description

CD2 is a 45-58 kD type I transmembrane glycoprotein, also known as LFA-2, T11 or Ly-37. It is a member of the Ig superfamily. Mouse CD2 is primarily expressed on T cells, B cells, thymocytes and NK cells. It is a ligand for CD48 and is involved in T cell activation and differentiation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of CD2-mediated cell-cell adhesion and inhibition of T cell activation1, blocking of T cell A.I.C.D.2, immunoprecipitation3, and co-induction of thymocyte maturation4. The RM2-5 antibody can block CD2-mediated cell-cell adhesion. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 100118 and 100119).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1990. J. Immunol. 145:3628. (Block)
  2. Ayroldi E, et al. 1997. Blood 89:3717. (Block)
  3. Criado G, et al. 1996. Eur. J. Immunol. 26:1228. (IP)
  4. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
Product Citations
  1. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  2. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  3. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  4. Hasenberg M, et al. 2011. PLoS One. 6:e17314. PubMed
  5. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
RRID
AB_312650 (BioLegend Cat. No. 100103)
AB_312651 (BioLegend Cat. No. 100104)

Antigen Details

Structure
Ig superfamily, 45-58 kD
Distribution

T cells, thymocytes, NK cells, B cells

Function
T cell activation, adhesion
Ligand/Receptor
CD48, lck
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis SJ, et al. 1996. Immunol. Today 17:177.
3. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.

Gene ID
12481 View all products for this Gene ID
UniProt
View information about CD2 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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