Biotin anti-mouse CD140a Antibody

Pricing & Availability
Clone
APA5 (See other available formats)
Regulatory Status
RUO
Other Names
PDGF receptor-α, PDGFR-α
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
APA5_Biotin_030510
Mouse fibroblast NIH/3T3 cells are stained with biotinylated APA5, followed by Sav-PE
  • APA5_Biotin_030510
    Mouse fibroblast NIH/3T3 cells are stained with biotinylated APA5, followed by Sav-PE
Cat # Size Price Quantity Check Availability Save
135909 25 µg $50.00
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135910 100 µg $140.00
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Description

Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PDGFR-α-hIgG1 recombinant fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Takakura N, et al. 1996. J. Invest. Dermatol. 107:770.
  2. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block)
  3. Chen H, et al. 2015. ASN Neuro 8:7. PubMed.
  4. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)
Product Citations
  1. Adam RC et al. 2018. Cell stem cell. 22(3):398-413 . PubMed
  2. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  3. Laurin M, et al. 2019. Elife. 8. PubMed
  4. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  5. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  6. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  7. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  8. Kantelhardt S, et al. 2010. PLoS One. 5:e11323. PubMed
  9. Larsen SB, et al. 2021. Cell Stem Cell. :. PubMed
RRID
AB_2043973 (BioLegend Cat. No. 135909)
AB_2043974 (BioLegend Cat. No. 135910)

Antigen Details

Structure
Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution

Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.

Function
Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand/Receptor
PDGFs
Cell Type
Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551
2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124
3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883

Gene ID
18595 View all products for this Gene ID
UniProt
View information about CD140a on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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