Biotin anti-mouse CD126 (IL-6Rα chain) Antibody

Pricing & Availability
Clone
D7715A7 (See other available formats)
Regulatory Status
RUO
Other Names
IL-6 Receptor α chain, IL-6R
Isotype
Rat IgG2b, κ
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Product Citations
publications
d7715a7
F0 mouse myeloma cell line stained with D7715A7 PE
  • d7715a7
    F0 mouse myeloma cell line stained with D7715A7 PE
Cat # Size Price Quantity Avail. Save
115803 50 µg $75.00
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Description

CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation. The D7715A7 (15A7) antibody blocks IL-6/IL-6 receptor interactions.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
OKT4 hybridoma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2,5in vivo receptor blocking1-2,4-6,8, and in vitro neutralization7. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115804) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115808).

Please note, this clone had previously reported reactivity to rat, however upon further testing it has been determined to not react with rat.

Application References

(PubMed link indicates BioLegend citation)
  1. Coulie PG, et al. 1990. Curr. Top. Microbiol. Immunol. 166:43. (Block, IP)
  2. Vink A, et al. 1990. J. Exp. Med. 172:997. (Block, IP)
  3. Sun R, et al. 2004. J. Immunol. 172:5648. (FC)
  4. McKinney WJ, et al. 1998. Am. J. Respir. Cell Mol. Biol. 18:696. (Block)
  5. Weigert C, et al. 2006. J. Biol. Chem. 281:7060. (Block, IP)
  6. Koizumi N, et al. 2007. J. Immunol. 178:1767. (Block)
  7. Walker F, et al. 2008. Blood 111:3978. (Neut) PubMed
  8. Yadav M, et al. 2008. J. Immunol. 180:2772. (Block) PubMed
  9. Sintes J, et al. 2010. J. Leukoc. Biol. 88:687. PubMed
Product Citations
  1. Verghese DA, et al. 2018. JCI Insight. 3:. PubMed
  2. Yang J, et al. 2019. Front Immunol. 2.492361111. PubMed
  3. Zuo D, et al. 2009. Int Immunol. 1.65625. PubMed
RRID
AB_313674 (BioLegend Cat. No. 115803)

Antigen Details

Structure
Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
Distribution

Activated T cells and B cells, monocytes, hepatocytes, plasma cells

Function
Stimulates T cells, B cells, hematopoietic precursor proliferation and differentiation
Ligand/Receptor
IL-6
Cell Type
B cells, Mesenchymal Stem Cells, Monocytes, Plasma cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taga T, et al. 1997. Annu. Rev. Immunol. 15:797.
2. Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London.
3. Boulanger MJ, et al. 2003. Science 300:2101.

Gene ID
16194 View all products for this Gene ID
UniProt
View information about CD126 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 11/08/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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