- 5H4 (See other available formats)
- Regulatory Status
- Other Names
- IL-2 Receptor β chain, IL-2Rβ
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.Product Details
- Antibody Type
- Host Species
- Rat myeloma YB2/0 transfected with truncated mouse Il-2Rβ cDNA
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2.
(PubMed link indicates BioLegend citation)
- Furse R, et al. 1993 Eur. J. Immunol. 23:3181. (IP)
- He YW, et al. 1995. J. Immunol. 154:1596. (IP)
- Liqons DL, et al. 2012. J Biol Chem. 287:34386. PubMed.
- Product Citations
AB_313226 (BioLegend Cat. No. 105903)
AB_313227 (BioLegend Cat. No. 105904)
- Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
T cells and B cells, NK cells, monocytes, macrophages
- Critical component of IL-2 and IL-15 signaling
- IL-2, IL-15
- Cell Type
- B cells, Macrophages, Monocytes, NK cells, T cells, Tregs
- Biology Area
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245.
3. Suzuki H, et al. 1995. Science 268:1472.
4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.
- Gene ID
- 16185 View all products for this Gene ID
- View information about CD122 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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