- 359-81-11 (See other available formats)
- Regulatory Status
- Other Names
- Lymphotoxin-α (LT- α), Tumor necrosis factor-β (TNF-β), Coley's toxin, Hemorrhagic factor, Necrosin, Natural killer cytotoxic factor (NKCF), Differentiation inducing factor (DIF), TNFSF-1
- Mouse IgG1, κ
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- Product Citations
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Lymphotoxin-α (LT-α), also known as tumor necrosis factor-beta (TNF-β), is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. LT-α possesses a signal peptide sequence and is a secreted protein; however, LT-α is also present on the surface of activated T, B and LAK cells as a complex with LT-β. Bioactive LT-α exists as a homotrimer.Product Details
- Antibody Type
- Host Species
- E. coli expressed, recombinant human LT-α.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection - Quality tested
ELISPOT Detection, ICFC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 µg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA or ELISPOT Detection1,2: The biotinylated 359-81-11 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 359-238-8 antibody (Cat. No. 503002/503004) as the capture antibody.
Flow Cytometry3: The fluorochrome-labeled 359-81-11 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify LT-α -producing cells within mixed cell populations. View intracellular cytokine staining protocol.
Neutralization1,2: The 359-81-11 antibody can neutralize the bioactivity of natural or recombinant LT-α. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human LT-α bioactivity (Cat. No. 503108).
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
(PubMed link indicates BioLegend citation)
- Meager A, et al. 1987. J. Immunol. Methods 104:31.
- Meager A, et al. 1987. Hybridoma. 6:305.
- Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
AB_315274 (BioLegend Cat. No. 503104)
- TNF superfamily; trimer; 25 kD (Mammalian).
- Transformed cell cytotoxicity; mediator of inflammatory and immune functions; fibroblast synthesis of GM-CSF, G-CSF, IL-1, collagenase, prostaglandin E2; monocyte terminal differentiation, synthesis of G-CSF; neutrophil chemoattractant, production of reactive oxygen, fibroblast proliferation.
- Cell Sources
- Activated T and B cells, fibroblasts, astrocytes, myeloma, endothelial cells, epithelial cells.
- Cell Targets
- Monocytes, B cells, fibroblasts, neutrophils, osteoclasts, keratinocytes, endothelial cells.
- TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75).
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Aggarwal, B., et al.Eds. 1992. Tumor necrosis factors:structure, function, and mechanism of action. Marcel Dekker Inc.
3. Bonavida, B., et al.Eds. 1990. Tumor necrosis factor:structure, mechanisms of action, role in disease and therapy. Karger, Basel.
4. Paul, N., et al. 1987. Annu. Rev. Immunol. 6:407.
- Type II integral membrane protein, forms heterotrimer with type II integral membrane protein LT-β either as LTα1β2 or LTα2β1; processed secreted form is trimeric.
- Gene ID
- 4049 View all products for this Gene ID
- View information about LT-alpha on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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