Biotin anti-human FGF-basic Antibody

Pricing & Availability
Clone
JKFb-2 (See other available formats)
Regulatory Status
RUO
Other Names
Fibroblast Growth Factor-basic, bFGF, FGF-2, Heparin-binding growth factor
Isotype
Mouse IgG1, κ
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Product Citations
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Cat # Size Price Quantity Avail. Save
508702 500 µg $355.00
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Description

Fibroblast growth factor-basic (FGF-b, FGF-2) is a heparin-binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-basic also exerts a potent angiogenic activity in vivo. FGF-basic has been isolated from neural, pituitary, adrenal cortex, and placental tissues. The JKFb-2 antibody reacts with human fibroblast growth factor - basic (FGF-basic).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human bFGF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human FGF-basic protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate.It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated JKFb-2 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified BHFB15 antibody (Cat. No. 514801/514802) as the capture antibody.

RRID
AB_315527 (BioLegend Cat. No. 508702)

Antigen Details

Structure
FGF; 17.2 kD (Mammalian)
Bioactivity
Modulator of cell proliferation, motility, differentiation, angiogenesis; affinity for heparin; associated with ECM
Cell Sources
Brain, retina, pituitary, kidney, placenta, testis, corpus luteum, adrenal glands, monocytes, prostate, bone, liver, cartilage, endothelial cells, epithelial cells
Cell Targets
Fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, astrocytes, oligodendrocytes, smooth muscle
Receptors
FGFR-1 (flg); FGFR-2 (bek, K-sam); FGFR-4 (flg-2); low affinity coreceptor heparin sulfate and heparin sulfate proteoglycans required for full activity
Biology Area
Angiogenesis, Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Cytokines/Chemokines, Growth Factors
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Klagsbrun M. 1992. Semin. Cancer Biol. 3:81.
3. Goldfarb M. 1990. Cell Growth Differ. 1:439.
4. Sensenbrenner M. 1993. Prog. Neurobiol. 41:683.

Regulation
Released after tissue injuries and during inflammatory processes
Gene ID
2247 View all products for this Gene ID
UniProt
View information about FGF-basic on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 11/20/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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