- J139G11 (See other available formats)
- Regulatory Status
- Other Names
- Mucosa-associated epithelial chemokine (MEC), CCK1, SCYA28, small inducible cytokine A28
- Mouse IgG2b, κ
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- Product Citations
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Human CCL28 was identified in an EST database using a human chemokine consensus sequence. CCL28 is constitutively expressed by mucosal epithelial cells such as exocrine glands, trachea, and colon. Acinar epithelial cells in human and mouse salivary glands are strongly positive for CCL28 by immunostaining, and human saliva and milk contain high concentrations of CCL28. Furthermore, it is known that human and mouse CCL28 have antimicrobial activity attributed to the C-terminus of CCL28 against Candida albicans, Gram-negative bacteria, and Gram-positive bacteria. In addition, using a CCR10 deficient mouse model, it was shown that CCR10 plays a critical role in localization and accumulation of IgA ASC in lactating mammary glands. Also, it has been described that CCL28 is upregulated in epithelial inflammation, and this upregulation allows the recruitment of Tregs expressing CCR10. In fact, a subpopulation of CCR10-expressing CD25+CD4+FOXP3+ Tregs with potent anti-inflammatory properties was isolated from a chronically inflamed human liver. Information suggests that tumor hypoxia promotes the recruitment of Tregs through induction of expression of CCL28, and this induction is mediated by the hypoxia inducible factor-1α (HIF-1α). Therefore, tumor hypoxia promotes tolerance and angiogenesis via CCL28 and Tregs.Product Details
- Antibody Type
- Host Species
- Recombinant human CCL28
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.1-0.5 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human CCL28 recombinant protein ranging from 1000 to 15.6 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection: The biotinylated J139G11 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified J132C3 antibody (Cat. No. 525602) as the capture antibody.
Note: For testing human CCL28 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human CCL28 ELISA Kit with Pre-coated Plates (Cat. No. 441207 & 441208) are specially developed and recommended.
AB_2563258 (BioLegend Cat. No. 525703)
Mucosal epithelial cells, primary human cholangiocytes
- CCL28 chemoattracts resting CD4, CD8, and IgA antibody secreting cells. It plays a role in extravasation of IgA ASC in the small intestine, colon, lactating mammary glands, and has antimicrobial activity. CCL28 is upregulated by IL-1 or n-butirate in human colon epithelial cell lines, and IL-1 and n-butirate have a synergistic effect on CCL28 upregulation. Hypoxia induces CCL28 in tumors.
- CD4 and CD8 T cells, IgA antibody secreting cells, CCR10-expressing CD25+ CD4+ Foxp3+ Tregs, CCR3 expressing eosinophils
- CCR10, CCR3
- Cell Type
- B cells, Eosinophils, Epithelial cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Antigen References
1. Wang W, et al. 2000. J. Biol. Chem. 275:22313.
2. Hieshima K, et al. 2003. J. Immunol. 170:1452.
3. Hieshima K, et al. 2004. J. Immunol. 173:3668.
4. Ogawa H, et al. 2004. Am. J. Physiol. Gastrointest. Liver Physiol. 287:G1062.
5. Eksteen B, et al. 2006. J. Immunol. 177:593.
6. Morteau O, et al. 2008. J. Immunol. 181:6309.
7. Liu B and Wilson E. 2010. Eur. J. Immunol. 40:186.
8. Facciabene A, et al. 2011. Nature 475:226.
- Gene ID
- 56477 View all products for this Gene ID
- View information about CCL28 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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