APC Rat IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK4530 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2b, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
400611 25 µg $40.00
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400612 100 µg $95.00
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Description

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The Ultra-LEAF™ purified antibody (Endotoxin <  0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 400643, 400644, 400671, 400672, 400675, and 400676) as negative control.

Application References

(PubMed link indicates BioLegend citation)
  1. Cervantes-Barragan L, et al. 2007. Blood 109:1131.
  2. Zeiser R, et al. 2007. Blood 109:2225.
  3. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
  4. Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
  5. Yi H, et al. 2009. Blood 113:5819. PubMed
  6. Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
  7. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
  8. Richards J, et al. 2015. Mol Cell Cardiol. 79:21. PubMed
Product Citations
  1. Wang X, et al. 2018. Cell Death Dis. 9:578. PubMed
  2. Nam GH, et al. 2018. Nat Commun. 9:2165. PubMed
  3. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  4. Rousset F, et al. 2018. Mol Ther Nucleic Acids. 14:351. PubMed
  5. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  6. Cañadas I, et al. 2018. Nat Med. 24:1143. PubMed
  7. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  8. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
  9. Schroder WA, et al. 2019. Sci Rep. 9:12421. PubMed
  10. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  11. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  12. Topper MJ et al. 2017. Cell. 171(6):1284-1300 . PubMed
  13. Harada Y, et al. 2019. Pain. 160:2050. PubMed
  14. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  15. Fu Y, et al. 2020. Sci Rep. 10:9027. PubMed
  16. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  17. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  18. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  19. Christoforou N, et al. 2013. PLoS One. 8:65963. PubMed
  20. Andersen S, et al. 2013. Cytokine. 64:54. PubMed
  21. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  22. Matsubara Y, et al. 2014. Sci Rep. 4:5043. PubMed
  23. Allman W, et al. 2016. PLoS One. 11: 0154518. PubMed
  24. Miki Y, et al. 2016. J Biol Chem. 291: 15588 - 15601. PubMed
  25. Mizuno T, et al. 2017. Sci Rep. 7:42714. PubMed
  26. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  27. Market M, et al. 2020. Front Immunol. 1.127083333. PubMed
  28. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  29. Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed
  30. Gu B, et al. 2020. Molecular Cell. 80(4):699-711.e7. PubMed
  31. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  32. Deng P, et al. 2021. Cell Stem Cell. 28(6):1057-1073.e7. PubMed
  33. Farhat AM, et al. 2021. Cell Reports. 35(4):109044. PubMed
  34. Marangoni F, et al. 2021. Cell. . PubMed
RRID
AB_326555 (BioLegend Cat. No. 400611)
AB_326556 (BioLegend Cat. No. 400612)

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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