APC/Cyanine7 anti-mouse TCR β chain Antibody

Pricing & Availability
Clone
H57-597 (See other available formats)
Regulatory Status
RUO
Other Names
TCR-β chain, TCR-β, β-TCR
Isotype
Armenian Hamster IgG
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Product Citations
publications
H57-597_APCCy7_TCRb_Antibody_FC_080113
C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) APC/Cyanine7 (filled histrogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
  • H57-597_APCCy7_TCRb_Antibody_FC_080113
    C57BL/6 mouse splenocytes were stained with TCR β (clone H57-597) APC/Cyanine7 (filled histrogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
See APC/Cyanine7 spectral data
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109219 25 µg $85.00
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109220 100 µg $195.00
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Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  2. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  3. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  4. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  5. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  6. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  7. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  8. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  9. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  10. Zhang LJ, et al. 2019. Immunity. 50:121. PubMed
  11. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  12. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  13. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  14. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  15. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  16. Cibrián D, et al. 2020. J Allergy Clin Immunol. 145:199. PubMed
  17. Jin C, et al. 2019. Cell. 176:998. PubMed
  18. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  19. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  20. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  21. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  22. Yabas M, et al. 2011. PLoS One. 6:e26440. PubMed
  23. Broeck T, et al. 2013. J Immunol. 190:1982. PubMed
  24. Dillon T, et al. 2013. Int Immunol. 25:259. PubMed
  25. Acker A, et al. 2014. PLoS One. 9:110015. PubMed
  26. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  27. Lei F, et al. 2012. J Vis Exp. 63: 3986. PubMed
  28. Hirata Y, et al. 2015. PLoS One. 10: 0141785. PubMed
  29. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  30. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  31. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  32. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  33. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  34. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  35. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  36. Devos M, et al. 2020. J Exp Med. 217:00:00. PubMed
  37. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  38. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  39. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  40. Xu K, et al. 2021. Immunity. 54(5):976-987.e7. PubMed
  41. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  42. Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed
  43. Giampazolias E, et al. 2021. Cell. . PubMed
  44. de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed
  45. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  46. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  47. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
RRID
AB_893626 (BioLegend Cat. No. 109219)
AB_893624 (BioLegend Cat. No. 109220)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/06/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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