APC/Cyanine7 anti-mouse CD48 Antibody

Pricing & Availability
Clone
HM48-1 (See other available formats)
Regulatory Status
RUO
Other Names
BCM1, SLAMF2, Blast-1
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
HM48-1_APCCy7_080412
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48-1) APC/Cyanine7 (filled histogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
  • HM48-1_APCCy7_080412
    C57BL/6 mouse splenocytes were stained with CD48 (clone HM48-1) APC/Cyanine7 (filled histogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103431 25 µg $110.00
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103432 100 µg $295.00
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Description

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
Product Citations
  1. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  2. Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
  3. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  4. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  5. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  6. Rebekka K Schneider et al. 2017. Cell stem cell. 20(6):785-800 . PubMed
  7. Passaro D et al. 2017. Cancer cell. 32(3):324-341 . PubMed
  8. Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed
  9. Giannini S, et al. 2020. Nat Commun. 0.705555556. PubMed
  10. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  11. Simon M, et al. 2019. Cell Metab. 29:871. PubMed
  12. Morales Torres C, et al. 2020. Nat Commun. 11:1792. PubMed
  13. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  14. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  15. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  16. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  17. De Koninck M, et al. 2020. Cell Reports. 32(6):108014. PubMed
  18. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  19. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  20. Naka K, et al. 2020. Nat Commun. 3.709027778. PubMed
  21. Naka K, et al. 2015. Nat Commun. 6: 8039. PubMed
  22. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  23. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  24. Lv K, et al. 2021. Cell Stem Cell. 28(7):1275-1290.e9. PubMed
RRID
AB_2561462 (BioLegend Cat. No. 103431)
AB_2561463 (BioLegend Cat. No. 103432)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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