APC anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
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Product Citations
publications
X54-57.1_1_APC_1_052912
C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD64 (clone X54-5/7.1) APC (top) or mouse IgG1, κ APC isotype control (bottom). Data shown was gated on the myeloid population.
  • X54-57.1_1_APC_1_052912
    C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD64 (clone X54-5/7.1) APC (top) or mouse IgG1, κ APC isotype control (bottom). Data shown was gated on the myeloid population.
  • X54-57.1_1_APC_2_052912
See APC spectral data
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139305 25 µg $90.00
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139306 100 µg $225.00
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
Product Citations
  1. Zhou Q, et al. 2014. J Immunol. 193:496. PubMed
  2. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  3. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  4. Terhorst D, et al. 2015. J Immunol. 195: 4953 - 4961. PubMed
  5. Qayum A, et al. 2016. J Immunol. 196: 4457 - 4467. PubMed
  6. Meliopoulos V, et al. 2016. PLoS Pathog. 12: 1005804. PubMed
  7. Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed
  8. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  9. Rothchild AC, et al. 2020. Bio Protoc. 10:00. PubMed
  10. Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed
  11. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  12. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  13. Clark JT, et al. 2021. eLife. 10:00. PubMed
  14. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  15. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  16. Stacy A, et al. 2021. Cell. 184(3):615-627.e17. PubMed
  17. Maisonneuve C, et al. 2021. Cell Reports. 34(4):108677. PubMed
  18. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  19. Earnest JT, et al. 2021. Cell Reports. 35(1):108962. PubMed
  20. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  21. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  22. Marangoni F, et al. 2021. Cell. . PubMed
  23. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  24. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  25. Steuerman Y et al. 2018. Cell systems. 6(6):679-691 . PubMed
  26. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  27. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  28. Zwick M, et al. 2019. Front Immunol. 10:222. PubMed
  29. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  30. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  31. de Tymowski C et al. 2019. Cell reports. 27(3):762-775 . PubMed
  32. Wang KC, et al. 2018. Immunohorizons. 2:407. PubMed
  33. Zhang S, et al. 2019. Mol Med Rep. 19:5377. PubMed
  34. Qi X, et al. 2019. Nat Commun. 10:2141. PubMed
  35. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  36. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  37. Yu‐Han Chang et al. 2017. Immunity. 47(5):943-958 . PubMed
  38. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
  39. Holly Bachus et al. 2018. Immunity. 50(1):225-240 . PubMed
  40. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  41. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
  42. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
  43. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  44. Huang WC, et al. 2019. Int J Hyperthermia. 36:1255. PubMed
  45. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  46. Joshi JC, et al. 2020. Cell Rep. 30:4096. PubMed
  47. Martin T, et al. 2018. Arch Toxicol. 92:323. PubMed
  48. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  49. Camara A, et al. 2019. Immunity. 50:1467. PubMed
  50. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  51. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  52. Sencio V, et al. 2020. Cell Reports. 30(9):2934-2947. PubMed
  53. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  54. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
RRID
AB_11219205 (BioLegend Cat. No. 139305)
AB_11219391 (BioLegend Cat. No. 139306)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD64
Specificity Alt (DOES NOT SHOW ON TDS):
CD64
UniProt
View information about CD64 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/14/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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