APC anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
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Product Citations
publications
104_APC_122607
C57BL/6 mouse splenocytes stained with 104 APC
  • 104_APC_122607
    C57BL/6 mouse splenocytes stained with 104 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
109813 25 µg $30.00
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109814 100 µg $105.00
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  2. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  3. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  4. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  5. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  6. Xu X, et al. 2018. Cell. 175:1336. PubMed
  7. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
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  12. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  13. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  14. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  15. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  16. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
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  19. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
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  24. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  25. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  26. Woodworth JS, et al. 2017. Mucosal Immunol. 0.802083333. PubMed
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  33. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  34. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  35. Takizawa H, et al. 2011. J Exp Med. 208:273. PubMed
  36. Markey K, et al. 2012. Blood. 119:5918. PubMed
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  38. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
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  42. Zhang J, et al. 2015. PLoS One. 10:130441. PubMed
  43. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  44. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  45. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  46. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  47. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  48. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  49. Kalinski AL, et al. 2020. Elife. 9:00. PubMed
  50. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  51. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
  52. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  53. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  54. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  55. Winkler ES, et al. 2020. Cell. 182(4):901-918.e18. PubMed
  56. Dufva O, et al. 2020. Cancer Cell. 38(3):380-399.e13. PubMed
  57. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  58. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  59. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  60. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  61. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  62. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  63. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  64. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  65. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  66. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
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RRID
AB_389210 (BioLegend Cat. No. 109813)
AB_389211 (BioLegend Cat. No. 109814)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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