APC anti-mouse CD25 Recombinant Antibody

Pricing & Availability
Clone
QA19A49 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, and Tac
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
QA19A49_APC_CD25_Recombinant_Antibody_062521.png
C57BL/6 mouse splenocytes were stained with anti-mouse CD4 Alexa Fluor® 488 and anti-mouse CD25 recombinant (clone QA19A49) APC (left) or rat IgG2a, κ isotype control APC (right).
  • QA19A49_APC_CD25_Recombinant_Antibody_062521.png
    C57BL/6 mouse splenocytes were stained with anti-mouse CD4 Alexa Fluor® 488 and anti-mouse CD25 recombinant (clone QA19A49) APC (left) or rat IgG2a, κ isotype control APC (right).
See APC spectral data
Cat # Size Price Quantity Avail. Save
162105 25 µg $85.00
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Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα. CD25 is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

This clone binds to mouse CD25, also known as the low affinity IL-2Rα.

RRID
AB_2894643 (BioLegend Cat. No. 162105)

Antigen Details

Structure
CD25 is a 55 kD glycoprotein.
Distribution

B cells, T cells, Thymocytes, Tregs

Function
IL-2 receptor
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/25/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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