APC anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_APC_080207
C57BL/6 mouse splenocytes stained with CD19 (clone 6D5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • 6D5_APC_080207
    C57BL/6 mouse splenocytes stained with CD19 (clone 6D5) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
See APC spectral data
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115511 25 µg $35.00
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115512 100 µg $105.00
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Wang X, et al. 2018. Mol Med Rep. 17:1660. PubMed
  2. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  3. Metidji A et al. 2018. Immunity. 49(2):353-362 . PubMed
  4. Johnson MO et al. 2018. Cell. 175(7):1780-1795 . PubMed
  5. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  6. Jasti S, et al. 2017. Biol Reprod. 96:134. PubMed
  7. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  8. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  9. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  10. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  11. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  12. Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed
  13. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
  14. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
  15. Rusconi M, et al. 2017. Sci Rep. 5.745138889. PubMed
  16. Fujita K, et al. 2019. Proc Natl Acad Sci U S A. 116:14714. PubMed
  17. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  18. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  19. Cao A, et al. 2018. Nat Commun. 9:3288. PubMed
  20. Aalipour A, et al. 2020. Mol Ther Oncolytics. 17:232. PubMed
  21. Wang W, et al. 2020. Immunity. 51(6):1102-1118.e7.. PubMed
  22. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  23. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  24. Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
  25. Olive V, et al. 2009. Genes Dev. 2.929861111. PubMed
  26. Ko S, et al. 2009. J Virol. 83:748. PubMed
  27. Bazett M, et al. 2012. J Immunol. 188:2297. PubMed
  28. Schmidt M, et al. 2012. J Virol. 86:11654. PubMed
  29. Horikoshi M, et al. 2012. PLoS One. 7:e51215. PubMed
  30. Olive V, et al. 2013. Elife. 2:822. PubMed
  31. Miyai T, et al. 2014. Proc Natl Acad Sci U S A. 111:1780. PubMed
  32. Ma L, et al. 2015. Blood. 126: 247 - 256. PubMed
  33. Liu J, et al. 2015. Sci Rep. 5: 13558. PubMed
  34. Cook KD, et al. 2015. Immunity. 43:703-714. PubMed
  35. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  36. Li S, et al. 2016. Sci Rep. 6:20425. PubMed
  37. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  38. Wigton E, et al. 2016. J Leukoc Biol. 100: 143 - 153. PubMed
  39. Zhang B, et al. 2016. Mol Cell. 63: 976-89. PubMed
  40. Knocke S, et al. 2016. Cell Rep. 17:2234-2246. PubMed
  41. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
  42. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  43. Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed
  44. Lewandowski JP, et al. 2020. Genome Biol. 1.039583333. PubMed
  45. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  46. Ligon MM, et al. 2020. Mucosal Immunol. 1.172222222. PubMed
  47. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
  48. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
  49. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  50. Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed
  51. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  52. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  53. Socodato R, et al. 2020. Sci Signal. 13: . PubMed
RRID
AB_313646 (BioLegend Cat. No. 115511)
AB_313647 (BioLegend Cat. No. 115512)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/24/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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