APC anti-mouse CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
281-2 (See other available formats)
Regulatory Status
RUO
Other Names
Syndecan-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
281-2_APC_1_120911
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) APC (top) or rat IgG2a, κ APC isotype control (bottom). Data shown is from gated on lymphoid cell population.
  • 281-2_APC_1_120911
    C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) APC (top) or rat IgG2a, κ APC isotype control (bottom). Data shown is from gated on lymphoid cell population.
  • 281-2_APC_2_120911
See APC spectral data
Cat # Size Price Quantity Avail. Save
142505 25 µg $130.00
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142506 100 µg $325.00
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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary gland epithelial cell line NMuMG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
  2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
  3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
  4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations
  1. Castiblanco D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01833. PubMed
  2. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  3. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  4. Chen F et al. 2018. Cell reports. 25(10):2775-2783 . PubMed
  5. Price MJ, et al. 2019. J Immunol. 203:2121. PubMed
  6. Xiong Y, et al. 2019. BMC Immunol. 20:24. PubMed
  7. Roy K, et al. 2019. Immunity. 50:616. PubMed
  8. Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed
  9. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  10. Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed
  11. Yao W, et al. 2019. Nature. 568:410. PubMed
  12. Lightman SM, et al. 2020. Cell Reports. 1.086805556. PubMed
  13. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  14. Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed
  15. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  16. Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed
  17. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  18. Alrefai H, et al. 2016. Nat Commun. 7:11724. PubMed
  19. Han S, et al. 2016. Arthritis Res Ther. 0.975. PubMed
  20. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  21. Zhang YN, et al. 2020. EBioMedicine. 56:102819. PubMed
  22. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  23. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
  24. Delgado-Benito V, et al. 2020. J Exp Med. 217:00:00. PubMed
  25. Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed
  26. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  27. Li Z, et al. 2021. Cell Metabolism. 33(1):78-93.e7. PubMed
RRID
AB_10960141 (BioLegend Cat. No. 142505)
AB_10962911 (BioLegend Cat. No. 142506)

Antigen Details

Structure
Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function
Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID
20969 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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