APC anti-human CXCL9 (MIG) Antibody

Pricing & Availability
Clone
J1015E10 (See other available formats)
Regulatory Status
RUO
Other Names
MIG-1, MIG
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
J1015E10_APC_CXCL9_Antibody_ICFC_080315
IFN-γ and TNF-α stimulated (overnight in the presence of monensin) human peripheral blood monocytes were fixed with the Fixation Buffer and permeabilized with Permeabilization Wash Buffer . Cells were then stained with CXCL9 (clone J1015E10) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
  • J1015E10_APC_CXCL9_Antibody_ICFC_080315
    IFN-γ and TNF-α stimulated (overnight in the presence of monensin) human peripheral blood monocytes were fixed with the Fixation Buffer and permeabilized with Permeabilization Wash Buffer . Cells were then stained with CXCL9 (clone J1015E10) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
See APC spectral data
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357905 25 tests $125.00
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357906 100 tests $285.00
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Description

Chemokine (C-X-C motif) ligand 9 (CXCL9), also known as monokine induced by gamma interferon (MIG), is a small cytokine belonging to the non-ELR (lacking a Glu-Leu-Arg motif in the N-terminal region) motif CXC chemokines. CXCL9 shares the CXCR3 with CXCL10 and CXCL11, and these ligands differ in their receptor-binding and activating properties; CXCL11 is more potent than CXCL10 and CXCL10 is more potent than CXCL9. Some studies have revealed that these ligands have redundant functions in vivo; other studies have demonstrated that the three CXCR3 ligands can collaborate and even compete with each other.

CXCL9 is mainly produced by activated macrophages and some cancer cells. Functioning as a chemoattractant, CXCL9 attracts CXCR3-expressing cells (such as a subset of circulating T cells, B cells, and natural killer cells) into inflammatory sites. Like other chemokines, CXCL9 is also able to elicit intracellular calcium flux and activate PI3K and MAPK. It participates in multiple immune and inflammatory responses by affecting cell growth, movement, or activation.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CXCL9.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2566033 (BioLegend Cat. No. 357905)
AB_2566034 (BioLegend Cat. No. 357906)

Antigen Details

Structure
A member of the alpha subfamily of inflammatory chemokines.
Distribution
Activated macrophages and some cancer cells.
Function
Attracts CXCR3-expressing cells, such as a subset of circulating T cells, B cells, and natural killer cells.
Ligand/Receptor
CXCR3
Cell Type
Macrophages, B cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Groom JR, et al. 2012. Immunity 37:1091.
2. Loetscher P, et al. 2001. J. Biol. Chem. 276:2986.
3. Jones D, et al. 2000. Blood 95:627.
4. Liao F, et al. 1995. J. Exp. Med. 182:1301.

Gene ID
4283 View all products for this Gene ID
UniProt
View information about CXCL9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 08/07/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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