- MHR73-11 (See other available formats)
- Regulatory Status
- Other Names
- CD180, Radio-protective 105-kD protein, RP105
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD180, also known as RP105, is a 105 kD member of the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages and dendritic cells. The TLR family's structural characteristics are critically involved in protein-protein signaling interactions leading to the activation of the innate immune system. Additionally, MD-1, a secretory protein, associates with CD180 (RP105) at the cell surface and this complex, in conjunction with TLR4, recognizes bacterial LPS. The human CD180 (RP105) gene maps to 5q12. The MHR73-11 antibody is able to activate B cells and to protect against irradiation- or dexamethasone-induced apoptosis.Product Details
- Human, Baboon, Cynomolgus, African Green, Rhesus
- Antibody Type
- Host Species
- Human RP105 complexed with mouse MD-1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: intracellular staining1, immunoprecipitation1, immunohistochemistry1 of acetone-fixed frozen tissue sections, B cell activation1, protection from apoptosis induced by radiation or dexamethasone, and induction of the costimulatory molecule CD80 (B7-1)1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312913 and 312914).
(PubMed link indicates BioLegend citation)
- Miura Y, et al. 1998. Blood 92:2815.
- Indrevaer RL, et al. 2013. J. Immunol. 191:3624. PubMed
AB_2783186 (BioLegend Cat. No. 312911)
AB_2783187 (BioLegend Cat. No. 312912)
- Member of the toll-like receptor family, 105 kD
B cells, monocytes/macrophages, and dendritic cells
- Signaling protein involved in innate immunity
- Cell Type
- B cells, Dendritic cells, Macrophages, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
1. Miura Y, et al. 1998. Blood 92:2815.
- Gene ID
- 4064 View all products for this Gene ID
- View information about CD180 on UniProt.org
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