Anti-human CD3 PE-Cy5/CD4 PE/CD8 FITC Cocktail Antibody

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Regulatory Status
Mouse IgG1 (all clones)
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    Human peripheral blood lymphocytes were stained with anti-human CD3 PECy5/CD4 PE/CD8 FITC cocktail.
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319001 50 tests $275.00
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CD3: UCHT1 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes.
CD4: RPA-T4 antibody reacts with CD4, a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages.
CD8: RPA-T8 antibody reacts with CD8a, a 32-34 kD type I glycoprotein. CD8a forms a heterodimer or homodimer with CD8b. CD8 also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (express almost exclusively CD8a homodimers).

Product Details
Technical data sheet

Product Details

Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
This reagent is a combination of PE/Cy5 conjugated UCHT1, PE conjugated RPA-T4 and FITC conjugated RPA-T8 at optimal concentration for three-color flow cytometric analysis.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
This product is guaranteed for nine months. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

This product has a shelf-life of 12 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product.

This product has a shelf-life of 12 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product.

FC - Quality tested

Recommended Usage

For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood.

Application Notes

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.

Single color controls are required to perform instrument compensation and are not included in the kit.  

AB_316003 (BioLegend Cat. No. 319001)

Antigen Details


T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes

Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
4. Center D, et al. 1996. Immunol. Today 17:476.
5. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID 916 View all products for this Gene ID 925 View all products for this Gene ID
View information about CD3/CD4/CD8 on

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Go To Top Version: 3    Revision Date: 12/03/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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