Alexa Fluor® 647 anti-Tyrosine Hydroxylase Antibody

Pricing & Availability
Clone
2/40/15 (See other available formats)
Other Names
Tyrosine 3-monooxygenase, tyrosine 3-hydroxylase
Isotype
Mouse IgG2a, κ
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Product Citations
publications
2slash40slash15_A647_Tyrosine_Hydroxylase_Antibody_1_022019.png
IHC staining of Alexa Fluor® 647 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded human Substantia Nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.2 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 2slash40slash15_A647_Tyrosine_Hydroxylase_Antibody_1_022019.png
    IHC staining of Alexa Fluor® 647 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded human Substantia Nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.2 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 2slash40slash15_A647_Tyrosine_Hydroxylase_Antibody_2_022019.png
    IHC staining of Alexa Fluor® 647 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded human Substantia Nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 2slash40slash15_A647_Tyrosine_Hydroxylase_Antibody_3_022019.png
    IHC staining of Alexa Fluor® 647 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 2slash40slash15_A647_Tyrosine_Hydroxylase_Antibody_4_022019.png
    IHC staining of Alexa Fluor® 647 anti-Tyrosine Hydroxylase antibody (clone 2/40/15) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with Hoechst, and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
818007 25 µg $130
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818008 100 µg $275
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Description

Tyrosine hydroxylase, also known as tyrosine 3-monooxygenase, is the enzyme that converts amino acid L-Tyrosine to L-3, 4-Dihydroxyphenylalanine (L-DOPA).  L-DOPA is a precursor to dopamine, which is then converted to norepinephrine and epinephrine (adrenaline).  Dopamine, norepinephrine and epinephrine are all important neurotransmitters.  Alterations in the activity of tyrosine hydroxylase may be involved in a number of neurological diseases such as Parkinson's and schizophrenia.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against purified Tyrosine Hydroxylase from rat Pheochromocytoma tumor.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.2 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

This antibody is less reactive in human tissue in Western Blot.

RRID
AB_2801154 (BioLegend Cat. No. 818007)
AB_2801155 (BioLegend Cat. No. 818008)

Antigen Details

Structure
Expected MW: 65 kD
Cell Type
Dopaminergic Neurons, Neural Stem Cells, Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells, Synaptic Biology
Molecular Family
Enzymes and Regulators, Presynaptic proteins
Gene ID
25085 View all products for this Gene ID
UniProt
View information about Tyrosine Hydroxylase on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/20/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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