Alexa Fluor® 647 anti-rat CD11b/c Antibody

Pricing & Availability
OX-42 (See other available formats)
Regulatory Status
Other Names
Rat C3bi receptor, Rat CR3 complement receptor, ITGAM, ITGAX
Mouse IgG2a, κ
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Product Citations
LOU rat splenocytes stained with OX-42 Alexa Fluor® 647
  • 1_OX-42_Alx647_021308
    LOU rat splenocytes stained with OX-42 Alexa Fluor® 647
  • 2_OX-42_A647_CD11b-c_Antibody_2_010419
    Rat frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of CD3 (clone 1F4) Alexa Fluor® 488 (blue), 10 µg/mL of CD11b/c (clone OX-42) Alexa Fluor® 647 (yellow), and 10 µg/mL of purified CD38 (clone 14.27) overnight at 4°C, followed by 5 µg/mL of anti-mouse IgG2b (clone RMG2b-1) Alexa Fluor® 594 (red) for two hours at room temperature. The image was captured by 10X objective.
See Alexa Fluor® 647 spectral data
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201814 100 µg $195.00
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The OX-42 antibody reacts with the CR3 complement (C3bi) receptor expressed on monocytes, granulocytes, macrophages, dendritic cells, and NK cells. This antibody appears to recognize a common epitope shared between CD11b and CD11c (integrin αM and αX chains). The OX-42 antibody precipitates three polypeptides with apparent molecular weights of 160, 103, and 95 kD, respectively. This antibody has been shown to block the formation of complement-mediated rosettes and leukocyte migration.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Rat peritoneal macrophages.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemistry staining on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of C3bi binding3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  2. Milligan CE, et al. 1991. J. Comp. Neurol. 314:125. (IHC)
  3. Robinson AP, et al. 1986. Immunology 57:239. (Block)
  4. Issekutz SE, et al. 1992. Immunology 76:655. (Block)
  5. Muehlbauer SM, et al. Am. J Pathol. 177:735. (FC) PubMed
  6. Thomas RA. et al. 2009. J Pharmacol Toxicol Methods. 60:263. PubMed
  7. Takeda Y, et al. 2011. Immunobiology. 216:1094. PubMed
  8. Furuhashi K, et al. 2013. J Am Soc Nephrol. PubMed
  9. Cheng HY, et al. 2013. Cytotherapy. 1465:608. PubMed
Product Citations
  1. Gounder AP, et al. 2018. PLoS Pathog. 14:e1007001. PubMed
  2. Rotem E, et al. 2018. PLoS Pathog. 14:e1007044. PubMed
  3. Raju S, et al. 2018. PLoS Pathog. 14:e1007053. PubMed
AB_2129391 (BioLegend Cat. No. 201814)

Antigen Details

Integrin family member

Monocytes, granulocytes, macrophages, dendritic cells

Receptor for C3bi
Complement C3bi
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Cell Biology, Complement, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Robinson AP, et al. 1986. Immunology 57:239.
2. Barcaly AN. 1981 Immunology 42:593.

Gene ID
25021 View all products for this Gene ID 499271 View all products for this Gene ID
View information about CD11b CD11c on

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Go To Top Version: 3    Revision Date: 01/04/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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