Alexa Fluor® 647 anti-mouse Podoplanin Antibody

Pricing & Availability
PMab-1 (See other available formats)
Regulatory Status
Other Names
T1a, gp38, stromal cell marker
Rat IgG2a, κ
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Product Citations
Mouse TE-71 cell line was stained with anti-mouse podoplanin (clone PMab-1) Alexa Fluor® 647 (filled histogram) or rat IgG2a, κ isotype control (clone RTK2758) Alexa Fluor® 647 (open histogram).
  • 1_PMab-1_A647_podoplanin_Antibody_092418
    Mouse TE-71 cell line was stained with anti-mouse podoplanin (clone PMab-1) Alexa Fluor® 647 (filled histogram) or rat IgG2a, κ isotype control (clone RTK2758) Alexa Fluor® 647 (open histogram).
  • 2_PMab-1_A647_Podoplanin_IHC-F_Antibody_091319
    C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10µg/ml of Alexa Fluor® 647 anti-mouse podoplanin (clone PMab-1, red), 10 µg/ml of Alexa Fluor® 594 anti-mouse CD326 (clone G8.8, green) at 4°C overnight. The image was scanned with a 10X object and stitched with MetaMorph® software.
  • 3_PMab-1_A647_Podoplanin_Antibody_3D-IHC_092121.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse kidney section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-Tubulin β 3 (TUBB3) Antibody (clone TUJ1) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse Podoplanin Antibody (clone PMab-1) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 20X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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See Alexa Fluor® 647 spectral data
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156203 25 µg $145
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156204 100 µg $305
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The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Peptide (38-51 amino acids of mouse podoplanin)
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC-F, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Yamada S, et al. 2017. Monoclon Antib Immunodiagn Immunother. 36(2):77-79.
AB_2750403 (BioLegend Cat. No. 156203)
AB_2750408 (BioLegend Cat. No. 156204)

Antigen Details

43 kD glycosylated type-1 transmembrane protein. Mucin-type protein.

Expressed on epithelial and mesothelial cells.

Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation.
Interacts with CLEC1B; the interaction is independent of CLEC1B glycosylation and activates CLEC1B; the interaction is dependent of sialic acid on O-glycans.
Cell Type
Epithelial cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Farr A, et al. 1992. J Histochem Cytochem. 40:651.
  2. Schacht V, et al. 2005. Am J Pathol. 166:913.
Gene ID
14726 View all products for this Gene ID
View information about Podoplanin on

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Go To Top Version: 2    Revision Date: 09/21/2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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